Abstract
Two small plasmids fromSelenomonas ruminantium strain 19D were cloned inEscherichia coli and completely characterized. Sequence comparison indicated that the plasmids are similar to those reported in genetically vaguely relatedS. ruminantium strain S20. Small 1.4-kb plasmids pSRD191 and pONE430 are only distantly related (≈30 % for deduced Rep protein amino acid sequence) but possess a short highly conserved region outsiderep gene. Larger plasmids pSRD192 and pONE429 possess large identical DNA regions in an otherwise dissimilar background. Recombination is proposed as an important mechanism of evolution and spreading ofS. ruminantium plasmids.
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This work was supported bySlovak Grant Agencies VEGA (grant no. 2/5140/25) and theSlovak Science and Technology Assistance Agency (grant no. APVT 5101 2602).
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Ivan, J., Šprincová, A., Javorský, P. et al. Spreading and mutability ofSelenomonas ruminantium plasmids. Folia Microbiol 51, 283–285 (2006). https://doi.org/10.1007/BF02931814
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DOI: https://doi.org/10.1007/BF02931814