Abstract
Conidia production ofBeauveria sp. strain LAG by solid-state fermentation (SSF) using blends of agro-industrial residues (residual potatoes and sugar-cane bagasse) was optimized with respect to cultivation conditions and the composition of substrate mixture in Erlenmeyer flasks and column-type bioreactor. With a blend of 60 % residual potatoes and 40 % sugar-cane bagasse the optimum conditions achieved were: incubation temperature 26 °C, initial substrate pH 6, inoculum concentration 107 conidia per g substrate; optimal initial moisture of the substrate was 70 % for Erlenmeyer flasks, in column-type bioreactor (with forced aeration) the optimal initial moisture of the substrate was 65 % with airflow of 60 mL/min. The highest production (1.07 × 1010 conidia per g dry substrate) was achieved after a 10-d fermentation. The conidia were used in laboratory assays againstThelosia camina andHylesia sp., caterpillars that are serious pests of mate plants. The mortality ofT. camina was >90 % 10 d after spraying caterpillars with 1 mL conidia suspension at a concentration 105–108/mL. ForHylesia sp., the mortality was 70 %, 7 d after immersion in the conidia suspension containing 108 conidia per mL. Therefore, theBeauveria sp. LAG can be considered to be an important biocontrol instrument in the prospect of the Integrated Pest Management for mate plants.
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Dalla Santa, H.S., Sousa, N.J., Brand, D. et al. Conidia production ofBeauveria sp. by solid-state fermentation for biocontrol ofIlex paraguariensis caterpillars. Folia Microbiol 49, 418–422 (2004). https://doi.org/10.1007/BF02931603
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DOI: https://doi.org/10.1007/BF02931603