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Extended-spectrum β-lactamase-conferring transferable resistance to different antimicrobial agents inEnterobacteriaceae isolated from bloodstream infections

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Abstract

Twenty (18.5 %) out of 108 clinical isolates of the familyEnterobacteriaceae responsible for bloodstream infection were extended-spectrum β-lactamase (ESBL)-positive in two screening tests, the double disk synergy test and theOxoid Combination Disk method. Eleven out of the 20 ESBL-positive isolates transferred oxyimino-β-lactam resistance toE. coli K12 C600 recipient strain with a frequency of 10−8–10−1 per donor cell. PCR analysis revealed that the majority of the transconjugants (9 of 11) express CTX-M-type β-lactamases. Donor strains and their transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-β-lactams but susceptible to clavulanic acid and carbapenems. Resistances to aminoglycosides, tetracycline and mercuric chloride were, in some cases, co-transferred with oxyimino-β-lactam resistance, suggesting that various resistance determinants were carried by the same conjugative plasmids.

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Abbreviations

BSI:

bloodstream infection

CFU:

colony-forming unit

DDST:

double disk-synergy test

Akc:

amikacin

Amc:

amoxicillin

Azt:

aztreonam

Cef:

cefotaxime

Cfz:

ceftazidime

Cla:

clavulanate

ESBL:

extended-spectrum β-lactamase

OBLR:

oxyimino-β-lactam resistance

OCDM:

Oxoid combination disk method

Cmp:

chloramphenicol

Cpd:

cefpodoxime

Ctx:

ceftriaxone

Gmn:

gentamicin

Imi:

imipenem

Mer:

meropenem

Nal:

nalidixic acid

Nor:

norfloxacin

Pip:

piperacillin

Sxt:

co-trimoxazole

Ttc:

tetracycline

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Authors and Affiliations

  1. Department of Microbiology, University of Medicine in Wroclaw, 50 368, Wroclaw, Poland

    R. Franiczek, B. Krzyžanowska, I. Dolna, G. Mokracka & K. Szufnarowski

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  1. R. Franiczek
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  2. B. Krzyžanowska
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  3. I. Dolna
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  4. G. Mokracka
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  5. K. Szufnarowski
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Correspondence to R. Franiczek.

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Franiczek, R., Krzyžanowska, B., Dolna, I. et al. Extended-spectrum β-lactamase-conferring transferable resistance to different antimicrobial agents inEnterobacteriaceae isolated from bloodstream infections. Folia Microbiol 50, 119–124 (2005). https://doi.org/10.1007/BF02931459

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  • DOI: https://doi.org/10.1007/BF02931459

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