Skip to main content
SpringerLink
Log in

Occurrence and transferability of β-lactam resistance inEnterobacteriaceae isolated inChildren's University Hospital in Bratislava

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Occurrence and transferability of β-lactam resistance in 30 multi-resistantEscherichia coli, Klebsiella spp.,Enterobacter spp.,Pantoea agglomerans, Citrobacter freundii andSerratia marcescens strains isolated from children between 0 and 3 years of age is presented. The strains were resistant to ampicillin (30), cefoxitin (22), cefotaxime (30), ceftriaxone (30), ceftazidime (30) and aztreonam (28), but susceptible to cefepime (30) and imipenem (26). Twenty-eight of 30 isolates possessed a transferable resistance confirmed by conjugation and isolation of 79–89-kb plasmids. The β-lactam resistance was due to production of β-lactamases and ceftazidime proved to be stronger β-lactamase inductor than ceftriaxone. Twenty-five clinical isolates expressed transferable extended spectrum β-lactamases, and chromosomally encoded AmpC β-lactamase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adam A.: β-Lactam antibiotics: their role in management of infections in children.Pediatr. Infect. Dis. J. 17, S4-S7 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Babálová M., Blahová J., Lešická-Hupková M., Králiková K., Krčméry V., Urgelová A., Baníková I.: Transferable resistance to cefotaxime and other antibiotics in nosocomial strains ofSerratia marcescens, Proteus mirabilis andEnterobacter cloacae.Antiinfect. Drugs Chemother. 13, 177–178 (1995).

    Google Scholar 

  • Bauernfeind A., Schneider I., Jungwirth R., Sahly H., Ullman U.: A novel type of AmpC β-lactamase, ACC-1, produced by aKlebsiella pneumoniae strain causing nosocomial pneumonia.Antimicrob-Agents Chemother. 43, 1924–1931 (1999).

    PubMed  CAS  Google Scholar 

  • Bermudes H., Jude F., Chaibi E.B., Arpin C., Bebear C., Labia R., Quentin C.: Molecular characterization of TEM-59 (IRT-17), a novel inhibitor-resistant TEM-derivated β-lactamase in a clinical isolate ofKlebsiella oxytoca.Antimicrob. Agents Chemother. 43, 1657–1661 (1999).

    PubMed  CAS  Google Scholar 

  • Bradford P.A., Urban C., Mariano N., Projan S.J., Rahal J.J., Bush K.: Imipenem resistance inKlebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC β-lactamase, and the lost of an outer membrane protein.Antimicrob. Agents Chemother. 41, 563–569 (1997).

    PubMed  CAS  Google Scholar 

  • Bujdáková H., Klimáčková J., Allerberger F., Moravčíková M., Bagová M., Hanzen J., Michálková-Papajová D., Dierich M.P., Kettner M.: Spectrum and transferability of β-lactam resistance in hospital strains ofEnterobacter isolated in Bratislava and Innsbruck.Internat. J. Antimicrob. Agents 16, 31–36 (2000).

    Article  Google Scholar 

  • Bujdáková H., Laušová A., Jankovičová S., Prodinger W.P., Kallová J., Milošovič P., Kettner M.: Study of β-lactam resistance in ceftazidime-resistant clinical isolates ofEnterobacteriaceae.Internat. J. Antimicrob. Agents 10, 135–141 (1998).

    Article  Google Scholar 

  • Bush K.: Is it important to identify extended-spectrum β-lactamase-producing isolates?Eur. J. Clin. Microbiol. Infect. Dis. 15, 361–364 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Chanal C., Sirot D., Romasyko J.P., Bret L., Sirot J.: Survey of prevalence of extended spectrum β-lactamases amongEnterobacteriaceae.J. Antimicrob. Chemother. 38, 127–132 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Garau J.: Clinical strategies for serious infections: a European perspective.Diagn. Microbiol. Infect. Dis. 31, 397–404 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Gazouli M., Tzouvelekis L.S., Vatopoulos A.C., Tzelepi E.: Transferable class C β-lactamases inEscherichia coli strains isolated in Greek hospitals and characterization of two enzyme variants (LAT-3 and LAT-4) closely related toCitrobacter freundii AmpC β-lactamase.J. Antimicrob. Chemother. 42, 419–425 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Giramonti K.M., Johnson-Robins L.A., Weatherwax D.L., Horgan M.J.: Reevaluting practice changes: return to standard precautions after an outbreak of multidrug-resistantKlebsiella oxytoca.J. Nurs. Care Qual. 14, 57–62 (2000).

    PubMed  CAS  Google Scholar 

  • Huber T.W., Thomas J.S.: Detection of resistance due to inducible β-lactamase inEnterobacter cloacae.J. Clin. Microbiol. 32, 481–486 (1994).

    Google Scholar 

  • Jacoby G.A.: Genetics of extended-spectrum β-lactamases.Eur. J. Clin. Microbiol. Infect. Dis. 1, 2–11 (1994).

    Article  Google Scholar 

  • Jarlier V., Nicolas M., Fournier G., Philippon A.: Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents inEnterobacteriaceae: hospital prevalence and susceptibility patterns.Rev. Infect. Dis. 10, 867–878 (1998).

    Google Scholar 

  • Jenks P.J., Hu Y.M., Daniel F., Mentar S., Livermore D.M.: Plasmid-mediated production of Class I (AmpC) β-lactamase by twoKlebsiella pneumoniae isolates from UK.J. Antimicrob. Chemother 35, 235–236 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Leblebicioglu H., Nas Y., Eroglu C., Sunbul M., Esen S., Gunaydin M.: Detection of extended spectrum β-lactamases inEscherichia coli andKlebsiella pneumoniae.J. Chemother. 11, 103–106 (1999).

    PubMed  CAS  Google Scholar 

  • Leibovici L., Paul M., Poznanski O., Samra Z., Konigsberger H., Pitlik S.D.: Monotherapy versus β-lactam-aminoglycoside combination treatment for Gram-negative bacteremia: a prospective, observational study.Antimicrob. Agents Chemother. 41, 1127–1133 (1997).

    PubMed  CAS  Google Scholar 

  • Lindberg F., Normark S.: Contribution of chromosomal β-lactamases to β-lactam resistance inEnterobacteria.Rev. Infect. Dis. 8, 292–304 (1986).

    Google Scholar 

  • Maniatis A.N., Trougakos I.P., Katsanis G., Palermos J., Maniatis N.A., Legakis N.J.: Changing patterns of bacterial nosocomial infections—a 9 year study in a general hospital.Chemotherapy 43, 69–76 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Martinez-Martinez L., Pascual A., Hernandez-Alles S., Alvarez-Diaz D., Suarez A.I., Tran J., Benedi V.J., Jacoby G.A.: Roles of β-lactamases and porins in activities of carbapenems and cephalosporins againstKlebsiella pneumoniae.Antimicrob. Agents Chemother. 43, 1669–1673 (1999).

    PubMed  CAS  Google Scholar 

  • Medeiros A.A.: Evolution and dissemination of β-lactamases accelerated by generations of β-lactam antibiotics.Clin. Infect. Dis. 24, S19–45 (1997).

    Google Scholar 

  • Neuwirth C., Labia R., Siebor E., Pechinot A., Madec S., Chaibi E.B., Kazmierczak A.: Characterization of TEM-56, a novel β-lactamase produced byKlebsiella pneumoniae clinical isolate.Antimicrob. Agents Chemother. 44, 453–455 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Neuwirth C., Siebor E., Lopez J., Pechinot A., Kazmierczak A.: Outbreak of TEM-24-producingEnterobacter aerogenes in an intensive care unit and dissemination of the extended spectrum β-lactamase to other members of the familyEnterobacteriaceae.J. Clin. Microbiol. 24, 76–79 (1994).

    Google Scholar 

  • Nicolas-Chanoine M.H.: Inhibitor-resistant β-lactamases.J. Antimicrob. Chemother. 40, 1–3 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Nuech-Inderbinen M.T., Kayser F.H., Hacler H.: Survey and molecular genetics of SHV β-lactamases inEnterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12.Antimicrob. Agents Chemother. 41, 943–949 (1997).

    Google Scholar 

  • Palucha A., Mickiewicz B., Gniadkowski M.: Diversification ofEscherichia coli expressing and SHV-type extended-spectrum β-lactamase (ESBL) during a hospital outbreak: emergence of an ESBL-hyperproducing strain resistant to expanded-spectrum cephalosporins.Antimicrob. Agents Chemother. 43, 393–396 (1999).

    PubMed  CAS  Google Scholar 

  • Palucha A., Mickiewicz B., Hryniewicz W., Gniadkowski M.: Concurrent outbreaks of extended-spectrum β-lactamase-producing organisms of the familyEnterobacteriaceae in Warsaw hospital.J. Antimicrob. Chemother. 44, 489–499 (1999).

    Article  PubMed  CAS  Google Scholar 

  • Pena C., Pujol M., Ardanuy C.: Epidemiology and successful control of a large outbreak due toKlebsiella pneumoniae producing extended-spectrum β-lactamases.Antimicrob. Agents Chemother. 42, 53–58 (1998).

    PubMed  CAS  Google Scholar 

  • Pithout J.D., Sanders C.C., Sanders W.E. Jr.: Antimicrobial resistance with focus on β-lactam resistance in Gram-negative bacilli.Am. J. Med. 103, 51–59 (1997).

    Article  Google Scholar 

  • Sanchez L., Ruiz N., Leranoz S., Vinas M., Puig M.: The role of outer membrane inSerratia marcescens intrinsic resistance to antibiotics.Microbiologia 13, 315–320 (1997).

    PubMed  Google Scholar 

  • Schindler P., Huber G.: Use of PADAC, a novel chomogenic β-lactamase substrate, for the detection of β-lactamase producing organisms and assay of β-lactamase inhibitors/inactivators, pp. 169–176 in U. Brodbeck (Ed.):Enzyme Inhibitors. Springer-Verlag, Weinheim (Germany) 1980.

    Google Scholar 

  • Schnumacher H., Skibsted U., Hansen D.S., Scheibel J.: Cefuroxime resistance inKlebsiella pneumoniae. Susceptibility to cefotaxime and ceftazidime despite production of ESBL.APMIS 105, 708–716 (1997).

    Article  Google Scholar 

  • Takahashi S., Nagano Y.: Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis.J. Clin. Microbiol. 20, 608–613 (1994).

    Google Scholar 

  • Wiedeman B., Dietz H.: Cefotaxime—unchanged antibacterial activity over years?Diagn. Microbiol. Infect. Dis. 22, 5–12 (1995).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Virology, Faculty of Science, Comenius University, 842 15, Bratislava, Slovakia

    H. Bujdáková, S. Jankovičová, J. Klimáčková, M. Moravčíková, D. Michálková-Papajová, J. Kallova & M. Kettner

  2. Microbiological Laboratory HPL, 84107, Bratislava, Slovakia

    J. Hanzen & P. Milošovič

  3. Bristol-Myers Squibb, 811 03, Bratislava, Slovakia

    A. Jakab

Authors
  1. H. Bujdáková
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Hanzen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Jankovičová
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Klimáčková
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Moravčíková
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Milošovič
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. Michálková-Papajová
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. J. Kallova
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. Jakab
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. M. Kettner
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Bujdáková.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bujdáková, H., Hanzen, J., Jankovičová, S. et al. Occurrence and transferability of β-lactam resistance inEnterobacteriaceae isolated inChildren's University Hospital in Bratislava. Folia Microbiol 46, 339–344 (2001). https://doi.org/10.1007/BF02815624

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02815624

Keywords

Search

Navigation