Abstract
Streptomyces globisporus strains with knockouts inlndF andlndL genes, previously identified as possibly encoding cyclases governing cyclization of the nascent oligoketide (‘polyketide’) chain during the biosynthesis of the antitumor angucycline landomycin E, were prepared. On combining the results of sequence analysis and HPLC of extracts from mutant strains,lndL was suggested to control the first cyclization-aromatization event andlndF to be responsible for the 3rd–4th ring formation.
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References
Běhal V.: Hybrid antibiotics.Folia Microbiol. 48, 17–25 (2003).
Kieser H.M., Bibb M.J., Buttner M.J., Chater K.F., Hopwood D.A.:Practical StreptomycesGenetics. The John Innes Foundation, Norwich (UK) 2000.
Fedorenko V., Basiliya L., Pankevych K., Dubitska L., Ostash B., Luzhetskyy A., Gromyko O., Kruegel H.: Genetic control of antitumor antibiotics biosynthesis in actinomycetes.Bull.Inst.Agr.Microbiol. 2, 27–31 (2000).
Fernandez-Lozano M.J., Remsing L.L., Quiros L.M., Braña A.F., Fernández E., Sanchez C., Mendez C., Rohr J., Salas J.A.: Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin byStreptomyces argillaceus.J.Biol.Chem. 275, 3065–3074 (2000).
Krohn K., Rohr J.: Angucyclines: total syntheses, new structures and biosynthetic studies of an emerging new class of antibiotics.Topics Curr.Chem. 188, 127–135 (1997).
Luzhetskyy A.M., Ostash B.O., Fedorenko V.O.: Intergeneric conjugationEscherichia coli-Streptomyces globisporus 1912 with using of integrative plasmid pSET152 and its derivative.Rus.J.Genet. 37, 1340–1347 (2001).
Matselyukh B., Polishchuk L., Rohr J.: Plasmid-induced synthesis of antibiotics inStreptomyces. Abstr. 38,Conf. Biology of Streptomyces, Ohrbeck (Germany) 1996.
Pankevych K., Kruegel H., Fedorenko V.: Cloning and sequencing of a putative positive transcription regulator gene of landomycin E biosynthetic gene cluster ofStreptomyces globisporus 1912.Visn.L’viv.Univ.Ser.Biol. 27, 97–105 (2001).
Rawlings B.: Biosynthesis of polyketides (other than actinomycete macrolides).Nat.Prod.Rep. 16, 425–484 (1999).
Sambrook J., Fristch E.F., Maniatis T.:Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor-New York 1989.
Shen B., Hutchinson C.R.: Deciphering the mechanism for the assembly of aromatic polyketides by a bacterial polyketide synthase.Proc.Nat.Acad.Sci.USA 63, 6600–6604 (1996).
Staunton J., Weissman K.J.: Polyketide biosynthesis: a millenium review.Nat.Prod.Rep. 18, 380–416 (2001).
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This work was supported by INTAS (International Association for the Promotion of Cooperation with Scientists from the New Independent States of the Former Soviet Union) grant no. 00-208 to the first author and no. 00-186 to the fourth author. The help of Dr. A. Braña (Universidad de Oviedo, Spain) in HPLC of landomycins is gratefully acknowledged.
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Ostash, B., Rebets, Y., Yuskevich, V. et al. Targeted disruption ofStreptomyces globisporus lndF andlndL cyclase genes involved in landomycin E biosynthesis. Folia Microbiol 48, 484–488 (2003). https://doi.org/10.1007/BF02931329
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DOI: https://doi.org/10.1007/BF02931329