Abstract
RIM1 is a nuclear gene of the yeastSaccharomyces cerevisiae coding for a protein with single-stranded DNA-binding activity that is essential for mitochondrial genome maintenance. No protein partners of Rim1p have been described so far in yeast. To better understand the role of this protein in mitochondrial DNA replication and recombination, a search for protein interactors by the yeast two-hybrid system was performed. This approach led to the identification of several candidates, including a putative transcription factor, Azf1p, and Mph1p, a protein with an RNA helicase domain which is known to influence the mutation rate of nuclear and mitochondrial genomes.
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This work was supported in part bySlovak Grant Agency VEGA (1/0006/03, 1/9153/02) and APVT (no. 20-003 902), grant fromHoward Hughes Medical Institute (55000327),Fogarty International Research Collaboration Award (1-R03-TW05654-01, CFDA no. 93934),Comenius University Grant (UK/3690/99), grants fromHuman Frontier, theBelgian National Fund for Scientific Research, theEuropean Commission, and theInter-University Poles of Attraction Program of the Belgian Government Office for Scientific, Technical, and Cultural Affairs.
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Kucejová, B., Foury, F. Search for protein partners of mitochondrial single-stranded DNA-binding protein Rim1p using a yeast two-hybrid system. Folia Microbiol 48, 183–188 (2003). https://doi.org/10.1007/BF02930953
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DOI: https://doi.org/10.1007/BF02930953