Abstract
The present report is focused on the study of participation of exogenous DNA in the process of postirradiation reparation of meristematic cells ofVicia faba primary roots. It is aimed at comparison of the positive reparative effect of isologous DNA with postirradiation action of heterologous DNA in its native, thermally denatured and DNAase-degraded forms, or DNA degraded by ultrasound, and with the effect of other biologically important macromolecules (RNA, histone, heparin, and dextran sulphate). For this purpose, the roots ofVicia faba irradiated by 150 r exposure were cultivated in solutions containing the above substances for an appropriate time interval. In squash slides both mitotic activity of the investigated cell population and frequency of postmetaphase chromosomal aberrations induced by radiation were evaluated. It was shown that a stimulation of cell division and reparation of chromosome damages were supported exclusively by isologous DNA. On the contrary, exogenously applied heterologous DNA increased postirradiation frequency of aberrations; maintenance of native structure of applied DNA was an essential condition for the above effect. Other macromolecules investigated on the course of postirradiation reparation ofVicia faba meristematic cells were without effect.
Abstract
Předložená práce se podrobněji zabývá účastí exogenní DNK v procesu postiradiační reparace meristematických buněk primárního kořeneVicia faba. Jejím cílem je porovnat positivní reparační účinek isologní DNK s postiradiačním p⫲sobením jednak heterologní DNK nativní, denatur vané teplem a degradované DNasou, resp. ultrazvukem, jednak s účinkem jiných, biologicky významných makromolekul jako je RNK, histon, heparin a dextransulfát. Za tímto účelem byly primární kořínkyVicia faba po ozáření 150 r pěstovány v roztocích uvedených substancí vždy příslušný časový interval. Ve squashových preparátech pak byla hodnocena mitotická aktivita studované buněčné populace a změny ve frekvenci postmetafázových chromosomových aberací, indukovaných zářením. Výsledky ukázaly, že stimulaci buněčného dělení a reparaci chromosomálních poruch napomáhá výlučně isologní DNK. Heterologní DNK, podaná exogenně, naproti tomu zvyšuje postiradiační frekvenci aberací; nezbytnou podmínkou pro uvedený efekt je zachování nativní struktury aplikované DNK. Ostatní v práci zkoušené makromolekuly neovlivnily v žádném směru pr⫲běh postiradiační reparace meristematických buněkVicia faba.
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Šlotová, J., Karpfel, Z. & Kubíčková, D. Contribution to the study on the reparative effect of exogenous DNA in the irradiated meristem ofVicia faba . Biol Plant 13, 69–78 (1971). https://doi.org/10.1007/BF02930750
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DOI: https://doi.org/10.1007/BF02930750