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Oxidative replication stress induced by long-term exposure to hydroxyurea in root meristem cells of Vicia faba

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Abstract

Key message

Low concentrations of hydroxyurea, an inhibitor of DNA replication, induced oxidative and replicative stress in root apical meristem (RAM) cells of Vicia faba.

Abstract

Plant cells are constantly exposed to low-level endogenous stress factors that can affect DNA replication and lead to DNA damage. Long-term treatments of Vicia faba root apical meristems (RAMs) with HU leads to the appearance of atypical cells with intranuclear asynchrony. This rare form of abnormality was manifested by a gradual condensation of chromatin, from interphase to mitosis (so-called IM cells). Moreover, HU-treated root cells revealed abnormal chromosome structure, persisting DNA replication, and elevated levels of intracellular hydrogen peroxide (H2O2) and superoxide anion (O2∙−). Immunocytochemical studies have shown an increased number of fluorescent foci of H3 histones acetylated at lysine 56 (H3K56Ac; canonically connected with the DNA replication process). We show that continuous 3-day exposure to low concentrations (0.75 mM) of hydroxyurea (HU; an inhibitor of DNA replication) induces cellular response to reactive oxygen species and to DNA replication stress conditions.

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The published article includes all datasets generated or analyzed during this study. The authors will be happy to share raw data on request.

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Funding

Work stated from the statutory funds of the University of Łódź.

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Authors and Affiliations

  1. Department of Cytophysiology, Faculty of Biology and Environmental Protection, University of Lodz, 90-236, Lodz, Poland

    Aneta Żabka, Natalia Gocek, Justyna Teresa Polit & Janusz Maszewski

Authors
  1. Aneta Żabka
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  2. Natalia Gocek
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  3. Justyna Teresa Polit
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  4. Janusz Maszewski
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Contributions

AŻ planned, carried out the experiments and prepared the final version of the article, JM and JTP equally contributed to acquisition of the results, NG performed the statistical analyses. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Aneta Żabka.

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Communicated by Zsuzsanna Kolbert.

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Żabka, A., Gocek, N., Polit, J.T. et al. Oxidative replication stress induced by long-term exposure to hydroxyurea in root meristem cells of Vicia faba. Plant Cell Rep 43, 87 (2024). https://doi.org/10.1007/s00299-024-03187-x

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