Abstract
A total of 22 strains of known species of the genusSchizosaccharomyces Lindner were evaluated by numerical taxonomy based on conventional identification tests. The results of numerical taxonomy were supplemented by a determination of activity of extracellular hydrolytic enzymes, especially 1,3-β-D-glucanase, RNAase and DNAase. All the strains tested were capable of utilizing the 1,4-capable of utilizing the 1,4-α-D-glucan tamarind. Study of life cycles of these organisms showed that extracellular hydrolytic enzymes were present mainly at the time of maturation of asci and release of their walls. Strains forming four-spore asci could be comprised in the single speciesSchizosaccharomyces pombe Lindner as two varieties:S. pombe var.pombe andS. pombe var.malidevorans. The two varieties differ in maltose fermentation. Urease is produced by all strains irrespective of the life cycle phase. A number of hydrolytic enzymes are not produced by the genusSchizosaccharomyces (e.g. amylolytic enzymes) despite the fact that oligomers of the maltose type are utilized. Species of the genusSchizosaccharomyces lack also xylanase, cellulases, mannase, and are incapable of degrading carrageenan and acid polysaccharides.
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Kocková-Kratochvílová, A., Sláviková, E., Zemek, J. et al. Hydrolytic activity in the genusSchizosaccharomyces lindner. Folia Microbiol 30, 443–451 (1985). https://doi.org/10.1007/BF02928754
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DOI: https://doi.org/10.1007/BF02928754