Summary
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(1)
The alanine-2-14C and35S-methionine requirements of cells ofBacillus cereus andBacillus megatherium do not increase during sporulation. There is, however, an increase in the incorporation of35S-cysteine.
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(2)
The period of increased incorporation of35S-cysteine corresponds to the appearance of refractive spores. Incorporation of45Ca (in the form of45CaCl2) into cells ofBacillus cereus does not commence until after these “young spores” have been formed. Gradual thermostabilization of the spores occurs during the latter period.
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(3)
The “resting spore” period in the developmental cycle ofBacillus cereus and other test bacilli is characterized by a higher cystine content of the proteins. The formation of a cystine-containing protein structure commences during the prespore period, about two hours before the commencement of thermostabilization.
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(4)
During germination of spores ofBacillus cereus,45Ca is a typical exudate component, while35S remains in the sediment.
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After disintegration of the spores, the cystine-containing protein structure is localized in the sediment fraction, which consists mainly of the spore envelopes.
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The possible significance of the cystine-containing protein structure in spores of bacilli is discussed.
Abstract
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(1)
Аланин-2-14C и35S-метионин требования клеток Bacillus cereus и Bacillus мегатерий не увеличивают в течение sporulation. Существу ет, однако, Увеличение включени я35S-цистеина.
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(2)
Период увеличился инкорпорации от35 S-цистеина соответствует Появление рефракции спор. Инкорпорация от45 Ca (в виде45 CaCl2) в Клетки Bacillus cereus не начаться до тех пор, пока после этих “young спор” были формируется. Постепенное из thermostabilization спор происходит в течение последнего периода.
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(3)
“resting спор” период развития цикла Bacillus cereus и другие испытания бацилл характеризуе тся в высших cystine содержание белков. Формирование cystine-структуры, содержащ ие белок начинается prespore в течение периода, около двух часа до начала thermostabilization.
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(4)
На прорастание спор о Bacillus cereus,45Ca является типичны м exudate компонент, в то врем я как остается в35S отложений.
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(5)
После распада споры, cystine-структуры, содержащ ие белок это местное в осадок фракция, которая состоит в основном из конвертов спор.
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(6)
Возможные значения cystine-содержащих протеин структуры споры из бацилл обсуждается.
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Previous papers were published under the title “Sporulation of Bacilli” I–VII.
The author wishes to thank Mrs. M.Součková for technical assistance.
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Vinter, V. Spores of microorganisms. Folia Microbiol 5, 217–230 (1960). https://doi.org/10.1007/BF02928153
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DOI: https://doi.org/10.1007/BF02928153