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25-bp long border sequence of-TDNA is sufficient for cleavage byvir D endonuclease ofAgrobacterium

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Abstract

Agrobacterium tumefaciens-induced tumor formation in plant cells involves transfer of a defined segment of DNA (T-DNA) from the bacterium into plant cells. This process is known to involvevir genes, 25-bp-long T-DNA border sequences and additional sequences adjacent to border sequences called overdrive sequences. It is also known that thevir D gene-encoded endonuclease causes cleavage at the border sequences. In this report we show that a synthetic 25-bp border sequence is sufficient for cleavage and that overdrive sequences are not necessary for the cleavage catalysed byvirD endonuclease.

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Authors and Affiliations

  1. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India

    Srinivasan

  2. Department of Microbiology, University of Washington, 98195, Seattle, WA, USA

    Srinivasan, M. F. Yanofsky & E. W. Nester

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  1. Srinivasan
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  2. M. F. Yanofsky
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  3. E. W. Nester
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Srinivasan, Yanofsky, M.F. & Nester, E.W. 25-bp long border sequence of-TDNA is sufficient for cleavage byvir D endonuclease ofAgrobacterium . J Genet 68, 161–170 (1989). https://doi.org/10.1007/BF02927858

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  • DOI: https://doi.org/10.1007/BF02927858

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