Skip to main content
SpringerLink
Log in

Bacteriocin production inAgrobacterium tumefaciens

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Agrobacterium tumefaciens C58 forms “plaques” during layer cultivation. The “plaques” were shown not to be caused by the presence of a temperate bacteriophage or by random contamination. The “plaques” and their central microcolonies were used to repeatedly isolate cultures producing an antibiotic substance against the original strainA. tumefaciens C58, other nopaline strains, some octopine strains ofA. tumefaciens and some strains of the related genusRhizobium. The substance is thus a bacteriocin; in analogy to agrocins 84 and D286 it was named agrocin C58. The agrocin is not inactivated by trypsin. Its production by strain C58 was found only on cultivation on solid but not liquid media. The producing isolate ofA. tumefaciens C58 (strain C58i2) contains neither plasmid pTiC58 nor the plasmid analogous to pAgK84 which controls the production of agrocin 84 inA. radiobacter K84.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adams M.H.:Bacteriophages. Interscience, New York 1959.

    Google Scholar 

  • Beardsley R.E., Bohan J., Daly J.: Antibiotic production and glycine resistance inAgrobacterium tumefaciens.J.Bacteriol.84, 1345 (1962).

    PubMed  CAS  Google Scholar 

  • Bernaerts M.J., DeLey J.: A biochemical test for crown gall bacteria.Nature197, 406 (1963).

    Article  CAS  Google Scholar 

  • Brandis H., Šmarda J.:Bakteriocine und bakteriocinähnliche Substanzen. G. Fischer Verlag, Jena 1971.

    Google Scholar 

  • Casse F., Boucher C., Julliet J.S., Michel M., Denarie J.: Identification and characterisation of large plasmids mRhizobium meliloti using agarose gel electrophoresis.J.Gen.Microbiol.113, 229 (1979).

    CAS  Google Scholar 

  • Cooksey D.A., Moore L.W.: High frequency spontaneous mutations to agrocin 84 resistance inAgrobacterium tumefaciens andA. rhizogenes.Physiol.Plant Pathol.20, 129 (1982).

    Google Scholar 

  • Ellis J.G., Kerr A.:Agrobacterium: genetic studies on agrocin 84 production and the biological control of crown gall.Physiol.Plant Pathol.15, 311 (1979).

    Article  Google Scholar 

  • Hendson M., Askjaer L., Thomson J.A., Van Montagu M.: Broad-host-range agrocin ofAgrobacterium tumefaciens.Appl.Environ.Microbiol.45, 1526 (1983).

    PubMed  CAS  Google Scholar 

  • Holmes B., Roberts P.: The classification, identification and nomenclature ofAgrobacteria.J.Appl.Bacteriol.50, 443 (1981).

    Google Scholar 

  • Kahl G., Schell J.S. (eds.):Molecular Biology of Plant Tumors. Academic Press, New York 1982.

    Google Scholar 

  • Konisky J.: Colicins and other bacteriocins with established modes of action.Ann.Rev.Microbiol.36, 125 (1982).

    Article  CAS  Google Scholar 

  • Langley L.A., Kado C.I.: Studies inAgrobacterium tumefaciens. Conditions for mutagenesis by N-methyl-N’-nitro-N-nitrosoguanidine and relationship ofA. tumefaciens mutants-to crowngall tumor induction.Mutat.Res.14, 277 (1972).

    CAS  Google Scholar 

  • Manasse R.J., Staples R.C., Granados R.R., Barnes E.G.: Morphological, biological, and physical properties ofAgrobacterium tumefaciens bacteriophages.Virology47, 375 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Moore L.W., Warren G.:Agrobacterium radiobacter strain 84 and biological control of crown gall.Ann.Rev.Phytopathol.17, 163 (1979).

    Article  Google Scholar 

  • Murphy P.J., Tate M.E., Kerr A.: Substituents at N6 and C-5’ control selective uptake and toxicity of the adenine-nucleotide bacteriocin, agrocin 84, inAgrobacteria.Eur.J.Biochem.115, 539 (1981).

    Article  PubMed  CAS  Google Scholar 

  • Nečásek J., Dusbábková J., Vlasák J.: Production of agrocin inAgrobacterium tumefaciens C58.15th Int.Congr.Genet., Abstr. Contrib. Papers, Part I, p. 417, New Delhi 1983.

  • Pühler A. (ed.):Molecular Genetics of the Bacteria —Plant Interactions. Springer-Verlag, Berlin 1983.

    Google Scholar 

  • Slota J.E., Farrand S.K.: Genetic isolation and physical characterisation of pAg84, the plasmid responsible for agrocin 84 production.Plasmid8, 175 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Spiers A.G.: Biological control ofAgrobacterium speciesin vitro.N.Z.J.Agric.Bes.23, 133 (1980).

    Google Scholar 

  • Stonier T.:Agrobacterium tumefaciens Conn. II. Production of an antibiotic substance.J.Bacteriol.79, 889 (1960).

    PubMed  CAS  Google Scholar 

  • Vervliet G., Holsters M., Teuchy H., van Montagu M., Schell J.: Characterisation of different plaque-forming and defective temperate phages inAgrobacterium strains.J.Gen. Virol.26, 33 (1975).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Breeding Theory, Institute of Experimental Botany, Czechoslovak Academy of Sciences, 370 05, České Budějovice, Czechoslovakia

    J. Nečásek, J. Dusbábková & J. Vlasák

Authors
  1. J. Nečásek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Dusbábková
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Vlasák
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nečásek, J., Dusbábková, J. & Vlasák, J. Bacteriocin production inAgrobacterium tumefaciens . Folia Microbiol 30, 353–358 (1985). https://doi.org/10.1007/BF02927590

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02927590

Keywords

Search

Navigation