Summary
Two delayed hypersensitivity Systems were used, i.e. tuberculin hypersensitivity evoked by BCG infection and hypersensitivity to diphtheria toxoid produced by immunization with specific precipitate in adjuvants. The ability of lymph node cells and spleen cells of hypersensitive guinea pigs to transfer skin and systemic hypergensitivity afterin vitro incubation with specific and nonspecific antigen was investigated.
A short-time contact of hypersensitive cells with the gpecific antigenin vitro deprives them, completely or partially, of the capacity to transfer hypersensitivity to normal recipients. At the game time pyrogenic gubstances are producedin vitro. In vitro desensitization connected with the formation of pyrogenic substances concerns to the game extent the transfer of sygtemic as well as of gkin hypersensitivity, in both systemic and local hypersensitivity transfers.
These results are discussed from the point of view of the mechanism of delayed hypersensitivity which is considered as a reaction of hypersensitive cells with antigen resulting in the production of intermediary biologically active products.
Abstract
лспольэовались две системы иоздней гиперсенситивности: гиперсентивность к туберкулину, выэываемая инϕекциеи BCG, и гиперсенситивпость к анатоксиу диϕтерии, которая соэдавалась снособность клеток лимϕатических уэлов и селеэенки гиперсенситивных морских свинок переносить кожную и общую типерсенеитивность после инкубации in vitro co специϕическим или неспециециϕическим антигеном.
Краткосрочный контакт гиперсенситивных клеток со снециϕическим антигеном тнвных клеток со снециϕическим антигеном in vitro частично илиполностыо лишает их способности переносить гиперсенситивность на номального реципиента. При этом in vitro наблюдется обраэование пирогенных веществ. Десенсибилилиэация in vitro сочтающаяся с обраэованием пирогенных веществ, в одинаковоймере относится и к переносу кожной гиперсенситивности и к иереносу общей гиперсенситивности.
Эти результаты обсуждаются с точки эрения механиэма поэднего типа гиперсенситивности — как реакция гиперсенситивных клеток с антигеном сочетающаяся с обраэовапием промежуточных продуктов, обладающих биологической активностью.
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Johanovský, J. Desensitization of delayed hypersensitive cells by Antigenin vitro . Folia Microbiol 5, 381–389 (1960). https://doi.org/10.1007/BF02927189
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DOI: https://doi.org/10.1007/BF02927189