Abstract
Delayed-type hypersensitivity (DTH) is a useful approach for evaluating cell-mediated immune responses associated with Th1 reactivity. The DTH reaction is divided into the afferent and efferent phases. During the afferent phase of this model, mice are typically immunized by subcutaneous injection with a specific hapten or antigen in its chemically reactive state and emulsified with an adjuvant. The efferent phase is typically initiated 5–12 days after sensitization, whereby the previously sensitized mice are challenged by either subcutaneous footpad injection or intradermal ear injection. The DTH response is evaluated 24 h post challenge. Here, we describe a common protocol for the induction and assessment of the DTH reaction in mice using keyhole limpet hemocyanin.
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Hemmi H, Yoshino M, Yamazaki H, Naito M, Iyoda T, Omatsu Y, Shimoyama S, Letterio JJ, Nakabayashi T, Tagaya H, Yamane T, Ogawa M, Nishikawa S, Ryoke K, Inaba K, Hayashi S, Kunisada T (2001) Skin antigens in the steady state are trafficked to regional lymph nodes by transforming growth factor-beta1-dependent cells. Int Immunol 13:695–704
Yoshino M, Yamazaki H, Nakano H, Kakiuchi T, Ryoke K, Kunisada T, Hayashi S (2003) Distinct antigen trafficking from skin in the steady and active states. Int Immunol 15:773–779
Yoshino M, Yamazaki H, Shultz LD, Hayashi S (2006) Constant rate of steady-state self antigen trafficking from skin to regional lymph nodes. Int Immunol 18:1541–1548
Dieli F, Sireci G, Salerno A, Bellavia A (1999) Impaired contact hypersensitivity to trinitrochlorobenzene in interleukin-4-deficient mice. Immunology 98:71–79
Brand DD, Latham KA, Rosloniec EF (2007) Collagen-induced arthritis. Nat Protocols 2(5):1269–1275
Engstrom L, Pinzon-Ortiz MC, Li Y, Chen S, Kinsley D, Nelissen R, Fine JS, Mihara K, Manfra D (2009) Characterization of a murine keyhole limpet hemocyanin (KLH)-delayed-type hypersensitivity (DTH) model: role for p38 kinase. Int Immunopharmacol 9:1218–1227
Akahira-Azuma M, Szczepanik M, Tsuji RF, Campos RA, Itakura A, Mobini N, McNiff J, Kawikova I, Lu B, Gerard C, Jordan S (2004) Early delayed-type hypersensitivity eosinophil infiltrates depend on T helper 2 cytokines and interferon-γ via CXCR3 chemokines. Immunology 111:306–317
Arthur JC, Lich JD, Ye Z, Allen IC, Gris D, Schneider M, Roney KE, O’Connor BP, Moore CB, Morrison A, Sutterwala FS, Koller BH, Bertin J, Liu Z, Ting JPY (2010) Cutting edge: NLRP12 controls dendritic and myeloid cell migration to affect contact hypersensitivity. J Immunol 185(8):4515–4519
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Allen, I.C. (2013). Delayed-Type Hypersensitivity Models in Mice. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1031. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-481-4_13
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DOI: https://doi.org/10.1007/978-1-62703-481-4_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-480-7
Online ISBN: 978-1-62703-481-4
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