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Study of antibody formation by the use of metabolic inhibitors

I. The Effect of 6-mercaptopurine on Specific Immune Reactions

ИЗУЧЕНИЕ ОБРАЭОВАНИЯ АНТИТЕЛ ПОД ДЕЙСТВИЕМ МЕТАБОЛИЧЕСКИХ ИНГИБТОРОВ

I. влняние б-меркаптопурина па елецϕические процессы иммунитета

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Summary

  1. (1)

    In adult individuals the response to bacterial antigen usually has the character of a secondary reaction and is not influenced even by large doses of 6-mercaptopurine. The effect of 6-mercapto-purine on antibody response was therefore studied in young rabbits, in which a primary reaction is produced. It was found that doses of 0.3 and 0.5 mg./ 100 g. body weight led to complete inhibition of the antibody reaction after the injection ofSalmonella paratyphi B andBrucella suis antigen.

  2. (2)

    The phase of antibody formation influenced by 6-mercaptopurine was determined by transferring isolated spleen cells mixed with antigenin vitro to young rabbits. In all the experiments, 6-mercaptopurine was administered to the recipients subcutaneously in doses of 0.5 mg./ 100 g. for five days. If the administration of 6-mercaptopurine was initiated immediately after the transfer of normal cells mixed with antigenin vitro, antibody formation was completely inhibited. If the transferred cells were isolated from the donor during the phase of antibody production, however, the same doses of 6-mercaptopurine failed to inhibit the antibody reaction. Antibody formation was likewise not inhibited when the administration of 6-mercaptopurine was not begun until 72 hours after the transfer of normal cells mixed with antigenin vitro. When 6-mercaptopurine was administered 24 and 48 hours after transfer of the cells the antibody response was completely inhibited. This part of the experiments shows that 6-mercaptopurine is effective during the inductive phase of antibody formation only.

  3. (3)

    When 6-mercaptopurine was administered to one-month-old rabbits over a period of 14 days, during which 60 ml. blood and 500×106 spleen cells from an adult donor were administered, tolerance to a skin homograft from the corresponding donor was not induced.

Abstract

  1. (1)

    у взрослых особей реакция на бактериальный антигеп имеет характер преимущественно вторичной реакции, а 6-меркаптопурин не окаэывает на нее влияния даже в больших дозах. Поэтому мы исследовали вопрос, влияет ли 6-меркаптопурип па реакцию обраэоваторых он выэывает первичную реакцию. Мы установили, что дозы в 0,3 и 0,5 мг/100 г веса тела выэывают полное торможение реакции обраэовання антител после влрыскивания антигенов Salmonella paratyphi B и Brucella suis.

  2. (2)

    Мы иселедовали вопрос, на какие этапы обраэовапия антител действует 6-меркаптопурин. Для этого мы полъэовалисъ переносом молодым крольчатам изолиых с антигеном in vitro. 6-меркаптопурин мы вводили реципиентам под кожу в количестве 0,5 мг/100 г веса тела-во всех опытах всегда в теченне 5 дней. Если введение 6-меркаптопурина пачипается пемедленно послеперепоса нормалъных клеток, смешапных с аптигеном in vitro, наблюдается полное угнетение обраэования антител. Однако те же доэы 6-меркаптопурина не тормоэят реакции обраэоапия антител, если перенести молодым животным клетки, выделенные в период продукции антител. Введение 6-меркаптопурина не тормоэит также образовапия антител, сели после переноса нормальных клеток, емешаппых с аптигепом in vitro, его введение начинается толъко череэ 72 часа после переноса клеток. Если 6-меркаптопурин водить через 24 или 48 час. После переноса клеток, реакция образования анител оказывается полностью заторможенной. Эта чаеть опытов Докаэывает, что 6-мркаптопурин действует только в течение индуктивной ϕаэы обраэования антител

  3. (3)

    Введение 1-месячным крольчатам 6-меркаптопурина в течение 14 дней, в течение которых им было введено 60 мл крови от взрослого донора и 500?106 клеток селезенки, не соэдавало толерантности к кожному гомотранснлантату от соответственного донора.

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  1. Department of Microbiology, Institute of Biology, Czechoslovak Academy of Sciences, Prague

    J. Šterzl

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Šterzl, J. Study of antibody formation by the use of metabolic inhibitors. Folia Microbiol 5, 364–373 (1960). https://doi.org/10.1007/BF02927187

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