Abstract
The uptake of Mn2+ bySaccharomyces cerevisiae at the expense of endogenous sources of energy-depends on the stage of culture development and is maximum in the middle of the exponential phase. The ability of cells to take up Mn2+ is related to the content of intracellular potassium at all stages of growth, to the content of ATP during the exponential phase and it is not related to the content of inorganic polyphosphates. The uptake is inhibited by oligomycin (25 μg/ml) by 50–85 % and under anaerobic conditions by 10–50 %, depending on the stage of growth, indicating the role of aerobic phosphorylation in the process. The uptake of Mn2+ is apparently associated with a hydrolysis of low-molecular weight polyphosphates and ATP, as well as with the exit of K+ from cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fuhrmann G. F., Rothstein A.: The transport of Zn2+, Co2+ and Ni2+ into yeast cells.Biochim. Biophys. Acta 163, 325 (1968).
Fuhrmann G. F.: Aktiver Transport divalenter Kationen in Bäckerhefe in Beziehung zum Stoffwechsel, insbesondere der Gärung. H. Lang und P. Lang. Bern, Frankfurt am Main 1973.
Fuhrmann G. F.: Transport of divalent cations inSaccharomyces cerevisiae. Proc. 4th Internat. Symp. on Yeasts, Part 1, H. 1, Vienna 1974.
Indge K. L.: Polyphosphates of the yeast cell vacuoles.J. Gen. Microbiol. 51, 447 (1968).
Kotel’nikova A. V., Zvyagil’skaya R. A.:Biochemistry of Yeast Mitochondria (in Russian). Nauka, Moscow 1973.
Kováč Z., Hrušovská E., Šmigáň P.: Oxidative phosphorylation in yeast. VII. Inhibition of oxidative phosphorylation and of respiratory enzyme synthesis by oligomycin in intact cells.Biochim. Biophys. Acta 205, 520 (1970).
Kulayev I. S., Krasheninikov I. A., Kokurina N. K.: Localization of inorganic polyphosphates and nucleotides in the mycelium ofNeurospora crassa (in Russian).Biokhimiya 91, 850 (1966).
Mansurova S. E. Yermakova S. A., Zvyagil’skaya R. A., Kulayev I. S.: Synthesis of inorganic poyrphosphate by mitochondria of the yeast-like fungusEndomyces magnusii, coupled to the activity of the respiratory chain (in Russian).Mikrobiologiya 44, 874 (1975).
Mansurova S. E., Yermakova S. A., Kulayev I. S.: Extramitochondrial energy-dependent synthesis of inorganic pyrophosphate in yeast (in Russian).Biokhimiya 41, 1716 (1976).
Okorokov L. A.,Lichko L. P.,Kulaev I. S.: Abstr. FEBS Symp. Biochemistry of Membrane Transport, P-148. Zürich 1976.
Okorokov L. A., Lichko L. P., Kadomtseva V. M., Kholodenko V. P., Titovsky V. I., Kulaev I. S.: Energy-dependent transport of manganese into yeast cells and distribution of accumulated ions.Eur. J. Biochem. 75, 373 (1977).
Rothstein A., Jennings D. H.: Active transport of Mg and Mn into yeast cells.J. Gen. Physiol. 41, 585 (1958).
Schön G., Bachofen R.: Der Einfluss des Sauerstoffpartialdrucks und der Lichtintensität auf den ATP-Spiegel in Zellen vonAthiorhodaceae.Arch. Microbiol. 73, 34 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Okorokov, L.A., Kadomtseva, V.M. & Titovskii, B.I. Transport of manganese intoSaccharomyces cerevisiae . Folia Microbiol 24, 240–246 (1979). https://doi.org/10.1007/BF02926455
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02926455