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Effect of temperature on α-amylase formation and DNA replication inBacillus subtilis

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Abstract

The effect of temperature on extracellular α-amylase synthesis and chromosomal and plasrnid DNA replication inBacillus subtilis A18 carrying plasmid pMI 10 was studied. The specific growth rate μ increased with elevated temperature up to 42.5°C, while the activities of α-amylase per population dry mass decreased. No obvious quantitative changes of14C-thymidine incorporation per dry mass increase and no basic differences in plasmid copy number in the range of temperatures from 25 to 40°C were found.

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Author notes

  1. J. Hao

    Present address: Academia Sinica, Institute of Genetics, Beijing, China

Authors and Affiliations

  1. Department of Enzyme Engineering, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague

    J. Hao, J. Pazlarová, M. Strnadová & J. Chaloupka

  2. Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague

    P. Tichý

Authors
  1. J. Hao
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  2. J. Pazlarová
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  3. M. Strnadová
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  4. P. Tichý
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  5. J. Chaloupka
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Additional information

The authors are grateful to Dr. J. Hochmannová, Dr. J. Nešvera, Dr. M. Dvořáková and Dr. H. Viet for their help and valuable discussions.

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Hao, J., Pazlarová, J., Strnadová, M. et al. Effect of temperature on α-amylase formation and DNA replication inBacillus subtilis . Folia Microbiol 33, 230–233 (1988). https://doi.org/10.1007/BF02925910

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  • DOI: https://doi.org/10.1007/BF02925910

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