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Phenolic accumulation and peroxidase activity inin vitro selected alfalfa callus cultures resistant to filtrate ofFusarium spp.

  • Published:
Biologia Plantarum

Abstract

Changes in the phenylalanine ammonia-lyase (PAL) activity, accumulation of phenolic acids and ionically-bound peroxidase activity in thein vitro selected embryogenic and nonembryogenicMedicago sativa callus cultures resistant to the filtrate ofFusarium spp. were found. The PAL activity in bothin vitro selected cultures during a 4-week cultivation on a medium with phytotoxins was higher than in the control calli grown on a medium without toxin. The filtrate fromFusarium spp. evoked an increase in the contents of all determined phenolic acids in the selected calli. They occurred predominantly bound as esters. The most pronounced portions in the elevated acids level were of ester-bound p-hydroxybenzoic, vanillic, p-coumaric and ferulic acids. The ionic cell wall-bound peroxidase activity in both selected calli cultivated on a medium with a filtrate was twice as high as the activity determined in the control cultures. The activity of soluble peroxidase was not influenced by challenge with a filtrate. No significant differences were found between thein vitro selected embryogenic and nonembryogenic alfalfa callus cultures in the response to the phytotoxic filtrate.

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Abbreviations

EC:

embryogenie callus culture

NEC:

nonembryogenic callus culture

PAL:

L-phenylalanine ammonia-lyase

AA:

anisic acid

CA:

cinnamic acid

pCA:

p-coumaric acid

FA:

ferulic acid

pHBA:

p-hydroxybenzoic acid

SaA:

salicylic acid

SiA:

sinapic acid

VA:

vanillic acid

PhA:

phenolic acids

HPLC:

High-Performance Liquid Chromatography

FM:

fresh mass

References

  • Bajaj, K.L., Singh, P.P., Kaur, G.: Effect of cercosporin toxin on polyphenol metabolism in mungbean (Vigna radiata L. Wilczek) leaves. - Biochem. Physiol. Pflanzen180: 621–624, 1985.

    CAS  Google Scholar 

  • Beaudoin-Eagan, L.D., Thorpe, T.A.: Turnover of shikimate pathway metabolites during shoot initiation in tobacco callus cultures. - Plant Cell Physiol.25: 913–921, 1984.

    CAS  Google Scholar 

  • Binarová, P., Novák, J.F.: Studies on embryogenesis into different types of cell cultures ofMedicago sativa. - Acta Univ. Agr. (Brno)23: 293–296, 1985.

    Google Scholar 

  • Binarová, P., Nedělník, J., Fellner, M., Nedbálková, B.: Selection for resistance to filtrates ofFusarium spp. in embryogenic cell suspension culture ofMedicago sativa L. - Plant Cell Tissue Organ Cult.22: 191–196, 1990.

    Article  Google Scholar 

  • Blaydes, D.F.: Interaction of kinetin and various inhibitors of the growth of soybean tissue. - Physiol. Plant.19: 748–753, 1966.

    Article  CAS  Google Scholar 

  • Bolwell, G.P., Robbins, M.O., Dixon, R.A.: Metabolic changes in elicitor-treated bean cells. Enzymic responses associated with rapid changes in cell wall components. - Eur. J. Biochem.148: 571–578, 1985.

    Article  PubMed  CAS  Google Scholar 

  • Bray, H.C., Thorpe, W.V.: Determination of phenols. - In: Glick, D. (ed.): Methods of Biochemical Analysis. Vol. 1. Pp. 27–52. Academic Press, New York 1954.

    Chapter  Google Scholar 

  • Castillo, F.J., Penel, C, Greppin, H.: Peroxidase release induced by ozone inSedum album leaves. -Plant Physiol.74: 846–851, 1984.

    PubMed  CAS  Google Scholar 

  • Cvikrová, M., Hrubcová, M., Meravý L., Pospíšil, F.: Changes in the content of phenolic substances during the growth ofNicotiana tabacum cell suspension culture. - Biol. Plant.30: 185–192, 1988.

    Article  Google Scholar 

  • Dixon, R.A.: The phytoalexin response: Elicitation, signalling and the control of host gene expression. - Biol. Rev.61: 239–291, 1986.

    Article  CAS  Google Scholar 

  • Ebel, J.: Phytoalexin synthesis: The biochemical analysis of the induction process. - Annu. Rev. Phytopathol.24: 235–264, 1986.

    Article  CAS  Google Scholar 

  • Fanner, E.E.: Effects of fungal elicitor on lignin biosynthesis in cell suspension cultures of soybean. - Plant Physiol.78: 338–342, 1985.

    Google Scholar 

  • Ferraris, L., Abbattista Gentile, I., Matta, A.: Variations of phenols concentration as a consequence of stresses that induce resistance toFusarium wilt of tomato. - J. Plant Protect.94: 624–629, 1987.

    CAS  Google Scholar 

  • Fry, S.C.: Polymer-bound phenol as natural substrate of peroxidases. - In: Greppin, H., Penel, C., Gaspar, T. (ed.): Molecular and Physiological Aspects of Plant Peroxidases. Pp. 169–182. Université de Genève, Genève 1986.

    Google Scholar 

  • Gaspar, T., Penel, C., Greppin, H.: Peroxidases: Structures and catalytic reactions, biosynthesis, transport and location, physiological roles. - Bull. Group Polyphenols13: 159–176, 1986.

    CAS  Google Scholar 

  • Harkin, J.M., Obst, J.R.: Lignification in trees: indication of exclusive peroxidase participation. - Science180: 296–297, 1973.

    Article  PubMed  CAS  Google Scholar 

  • Jorrin, J., Dixon, R.A.: Stress responses in alfalfa (Medicago sativa L.): II. Purification, characterization, and induction of phenylalanine ammonia-lyase isoforms from elicitor-treated cell suspension cultures. - Plant Physiol.92: 447–455, 1990.

    PubMed  CAS  Google Scholar 

  • Kurosaki, F., Tashiro, N., Nishi, A.: Induction of chitinase and phenylalanine ammonia-lyase in cultured carrot cells treated with fungal mycelial walls. - Plant Cell Physiol.27: 1587–1591, 1986.

    CAS  Google Scholar 

  • Latunde-Dada, A.O., Lucas, J.A.: Influence of temperature on host resistance and fungal sensitivity to medicarpin in lucerne callus lines infected withVerticillium albo-atrum. - Physiol. mol. Plant Pathol.28: 89–97, 1986.

    CAS  Google Scholar 

  • Latunde-Dada, A.O., Dixon, R.A., Lucas, J.A.: Induction of phytoalexin biosynthetic enzymes in resistant and susceptible lucerne callus lines infected withVerticillium albo-atrum. - Physiol. mol. Plant Pathol.31: 15–23, 1987.

    Article  CAS  Google Scholar 

  • Legrand, M., Frilig, B., Hirth, L.: Enzymes of the phenylpropanoid pathway and the necrotic reaction of hypersensitive tobacco to tobacco mosaic virus. - Phytochemistry15: 1353–1359, 1976.

    Article  CAS  Google Scholar 

  • Mato, M.C., Rua, M.L., Ferro, E.: Changes in levels of peroxidases and phenolics during root formation inVitis culturedin vitro. - Physiol. Plant.72: 84–88, 1988.

    Article  CAS  Google Scholar 

  • Mayer, A.M.: Polyphenol oxidases in plants-recent progress. - Phytochemistry26: 11–20, 1987.

    Article  Google Scholar 

  • Mayer, A.M., Harel, E.: Polyphenol oxidase in plants. - Phytochemistry18: 193–215, 1979.

    Article  CAS  Google Scholar 

  • Meravy, L.: The effect of calcium deficiency on the activity of ammonia-lyases in the shikimic pathway. - Biol. Plant.21: 427–433, 1979.

    Article  CAS  Google Scholar 

  • Nedělník, J.: The production of phytotoxic metabolies ofFusarium oxysporum andFusarium solani in liquid media.- Ochr. Rost. (Praha)25: 261–269, 1989. [In Czech.]

    Google Scholar 

  • Niemann, G.J., Baayen, R.P.: Phenol metabolism and resistance againstFusarium oxysporum f.sp. dianthi inDianthus carophyllus. - Bull. Liaison Groupe Polyphenols14: 185–188, 1988.

    CAS  Google Scholar 

  • Pascholati, S.F., Heim, D., Nicholson, R.L.: Phenylalanine ammonia-lyase and susceptibility of the maize mesocotyl toHelminthosporium maydis. - Physiol. Plant Pathol.27: 345–356, 1985.

    Article  CAS  Google Scholar 

  • Van Loon, L.C., Geelem, J.L.M.C.: The relation of polyphenoloxidase and peroxidase to symptom expression in tobacco var. “Samsun NN” after infection with tobacco mosaic virus. - Acta phytopathol. Acad. Sci. hung.6: 9–20, 1971.

    Google Scholar 

  • Van Loon, L.C.: The significance of changes in peroxidase in diseased plants. - In: Greppin, H., Penel, C., Gasper, T. (ed.): Molecular and Physiological Aspects of Plant Peroxidases. Pp. 405–418. Université de Genéve, Genéve 1986.

    Google Scholar 

  • Zádor, E., Köves, E., Szabo, M.: Phenolic materials in auxin heterotroph and autotroph (habituated) tobacco callus tissues. - Biochem. Physiol. Pflanzen180: 125–131, 1985.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na Karlovce 1, 160 00, Praha 6, Czechoslovakia

    M. Hrubcová, M. Cvikrová, L. Meravy & J. Eder

  2. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Sokolovská 6, 772 00, Olomouc, Czechoslovakia

    P. Binarova

Authors
  1. M. Hrubcová
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  2. M. Cvikrová
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  3. L. Meravy
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  4. J. Eder
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  5. P. Binarova
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Additional information

Acknowledgements: We thank RNDr. J. Nedělnfk, Research Institute of Forage Crops, Troubsko, for providing the phytotoxic filtrates ofFusarium spp. and Mrs. N. HataŠová for her excellent technical assistance and preparation of the manuscript.

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Hrubcová, M., Cvikrová, M., Meravy, L. et al. Phenolic accumulation and peroxidase activity inin vitro selected alfalfa callus cultures resistant to filtrate ofFusarium spp.. Biol Plant 34, 203–211 (1992). https://doi.org/10.1007/BF02925869

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  • DOI: https://doi.org/10.1007/BF02925869

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