Abstract
Conditions under which it is possible to induce auxotrophic mutants and DL-selenomethionine-resistant mutants inB. flavum by N-methyl-N’-nitro-N-nitrosoguanidine were determined. The yield of auxotrophic mutants was increased to 3% during mutagenesis in the first stage and to 1.5% in the second stage when using the enrichment-selective method with vancomycin. The optimal vancomycin concentration for inactivation of prototrophic cells growing in a minimal medium was 200 mg/L and the optimal time of treatment was 8 h. When testing the effect of three amino acid analogues (dl-ethionine,dl-selenomethionine and L-methionine sulfoximine) it was found thatB. flavum is sensitive todl-selenomethionine present in the minimal cultivation medium. Mutants resistant to 1 mg/mL of selenomethionine were isolated. Both isotope studies and measurement of growth indicate thatdl-ethionine also entersB. flavum cells, although its competition with endogenously synthesized methionine is not significant.
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The authors wish to thank Mr. V. Košťál for his technical assistance.
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Koníček, J., Koníčková-Radochová, M., Strnadová, M. et al. Optimal conditions for induction of certain mutation types inBrevibacterium flavum by N-Methyl-N’-nitro-N-nitrosoguanidine. Folia Microbiol 33, 337–343 (1988). https://doi.org/10.1007/BF02925841
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DOI: https://doi.org/10.1007/BF02925841