Abstract
Cytological and cytophotometric analysis of root tips of regenerated plants, derived from rhizome expiant ofKniphofia nelsonii andKniphofia uvaria, revealed marked difference in behaviour of chromosomes and level of 4C nuclear DNA content. Karyotypic stability could be retained in all 52 regenerants of K. nelsonii whereas inK. uvaria out of 75 regenerants analysed, 12 plants were exclusively diploid and the rest 63 plants were predominantly diploid comprising variable amount of aneuploid and tetraploid cells. Cytological data was further confirmed by nuclear DNA content estimation. Alteration in the structure of chromosomes could also be noted in 57 regenerants ofK. uvaria giving rise to two new karyotypes. The use of polysomatic tissue for securing variantsin vitro inK. uvaria has been suggested.
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Armstrong, C.L., Phillips, R.L.: Genetic and cytogenetic variation in plants regenerated from organogenic and friable embryogenic tissue cultures of maize. — Crop Sci.28: 363–369, 1988.
Battaglia, E.: Chromosome morphology and terminology. — Caryologia8: 178–187, 1955.
Cassells, A.C., Carney, B.F.: Adventitious regeneration inPelargonium xdomesticum bailey. — Acta Hort.212: 419–423, 1987.
Detrez, C., Sangwan, R.S., Sangwan-Norreel, B.S.: Phenotypic and karyotypic status ofBeta vulgaris plants regenerated from direct organogenesis in petiole culture. — Theor. appl. Genet.77: 462–468, 1989.
Evans, N.E., Foulger, D., Farrar, L., Risiott, R., Bright, S.W.J.: Variation in expiant derived potato clones over three tuber generations. — Euphytica35: 353–361, 1986.
Fish, N., Karp, A.: Improvements in regeneration from protoplasts of potato and studies on chromosome stability 1. The effect of initial culture media. — Theor. appl. Genet.72: 405–412, 1986.
Krikorian, A.D., Staicu, S.A., Kann, R.P.: Karyotype analysis of a Day lily clone reared from aseptically cultured tissues. — Ann. Bot.47: 121–131, 1981.
Larkin, P.J., Brettell, R.I.S., Ryan, S.A., Davies, P.A., Pallotta, M.A., Scowcroft, W.R.: Somaclonal variation: impact on plant biology and breeding strategies. — In: Zaitlin, M. (ed.): Biotechnology in Plant Science. Pp. 83–100. Academic Press, London 1985.
MacLeish, J., Sunderland, N.: Measurement of DNA in higher plants by Feulgen cytophotometry and chemical methods. — Exp. Cell Res.24: 527–540, 1961.
Murashige, M., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. — Physiol. Plant.15: 473–497, 1962.
Nayak, S., Sen, S.: Differential requirement for organogenesis in two species ofKniphofia. — Indian J. exp. Biol.28: 171–173, 1990.
Reish, B.: Genetic variability in regenerated plants. — In: Evans, D.A., Sharp, W.R., Ammirato, P.V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Vol. 1. Pp. 749–769. Macmillan, New York 1983.
Sen, S.: Polysomaty and its significance inLiliales. — Cytologia38: 737–761, 1973.
Sengupta, J., Sen, S.: Propagation of species ofPolygonatum in vitro. — Curr. Sci.56: 1287–1289, 1987.
Sokal, P.R., Rohlf, F.J.: Introduction to Biostatistics. — Freeman and Co., San Francisco 1973.
Van’t Hof, J.: Relationships between mitotic cycle duration, S-period duration and the average rate of DNA synthesis in the root meristem cells of several plants. — Exp. Cell Res.39: 48–58, 1965.
Van Harten, A.M., Bouter, H., Broertjes, G.:In vitro adventitious bud techniques for vegetative propagation and mutation breeding of potato (Solatium tuberosum L.). II Significance for mutation breeding. — Euphytica30: 1–8, 1981.
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Nayak, S., Sen, S. Karyological and cytophotometric study of expiant derived clones of non-polysomatic and polysomatic species ofKniphofia . Biol Plant 34, 135–141 (1992). https://doi.org/10.1007/BF02925807
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DOI: https://doi.org/10.1007/BF02925807