Skip to main content
SpringerLink
Log in

Effects of gastric fundectomy and antrectomy on the exocrine pancreas in the hamster

  • Published:
International journal of pancreatology Aims and scope Submit manuscript

Summary

The effect of gastric fundectomy and antrectomy on growth of the exocrine pancreas was studied in hamsters over 5 and 25 d. Sham-operated animals served as controls. After 5 d, basal plasma gastrin concentrations were significantly increased in fundectomized animals (80.3± 20.6 prnol/L) and significantly decreased in antrectomized animals (11.6± 1.1 pmol/L) as compared with the controls (20.0 ± 1.7 pmol/L). Similar differences were present among the 25-d groups, whereas basal plasma cholecystokinin (CCK) concentrations did not differ significantly between any groups at any time. At 5 d after fundectomy, there was a significant increase in pancreatic tissue [3H]-thymidine uptake and total DNA content, both of which were reduced 5 d after antrectomy. Autoradiography showed significantly increased [3H]-thymidine labeling index of acinar, intralobular duct, and centroacinar cells of the pancreas at 5 d after fundectomy. The increased intralobular duct cell labeling index persisted 25 d after fundectomy. Labeling indexes after antrectomy did not differ significantly from those in the controls, although antrectomized animals had the lowest values in all three cell compartments at 25 d. At 25 d, pancreatic wet wt and total DNA and protein content were significantly increased after fundectomy and significantly reduced after antrectomy. These findings indicate that fundectomy in the hamster induces pancreatic exocrine tissue hyperplasia and hypertrophy, whereas antrectomy leads to retardation of pancreatic growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Reber HA, Johnson F, Deveney K, Montgomery C, Way LW. Trophic effects of gastrin on the exocrine pancreas in rats.J Surg Res 1911; 22: 554–560.

    Article  Google Scholar 

  2. Balas D, Senegas-Balas F, Pradayrol L, Vayssette J, Bertrand C, Ribet A. Long-term comparative effect of cholecystokinin and gastrin on mouse stomach, antrum, intestine, and exocrine pancreas.Am J Anat 1985; 174: 27–43.

    Article  PubMed  CAS  Google Scholar 

  3. Dembinski AB, Johnson LR. Stimulation of pancreatic growth by secretin, caerulein, and pentagastrin.Endocrinology 1980; 106: 323–328.

    Article  PubMed  CAS  Google Scholar 

  4. Solomon TE, Morisset J, Wood JG, Bussjaeger LJ. Additive interaction of pentagastrin and secretin on pancreatic growth in rats.Gastroenterology 1987; 92: 429–435.

    PubMed  CAS  Google Scholar 

  5. Johnson LR. Effects of gastrointestinal hormones on pan- creatic growth.Cancer 1981; 47: 1640–1645.

    Article  PubMed  CAS  Google Scholar 

  6. Dembinski AB, Johnson LR. Growth of pancreas and gastrointestinal mucosa in antrectomized and gastrin- treated rats.Endocrinology 1979; 105: 769–773.

    PubMed  CAS  Google Scholar 

  7. Scemama JL, Fourmy D, Zahidi A, Pradayrol L, Susini C, Ribet A. Characterization of gastrin receptors on a rat pancreatic acinar cell line (AR42J). A possible model for studying gastrin mediated cell growth and proliferation.Gut 1987; 28(Suppl. 1): 233–236.

    Article  PubMed  CAS  Google Scholar 

  8. Yu DH, Noguchi M, Zhou ZC, Villanueva ML, Gardner JD, Jensen RT. Characterization of gastrin receptors on guinea pig pancreatic acini.yAm J Physiol 1987; 253: G793-G801.

    CAS  Google Scholar 

  9. Oscarson J, Håkanson R, Liedberg G, Lundquist G, Sundler F, Thorell J. Variated serum gastrin concentration: trophic effects on the gastrointestinal tract of the rat.Acta Physiol Scand 1979; 475(Suppl.): 3–27.

    Google Scholar 

  10. Håkanson R, Blom H, Carlsson E, Larsson H, Ryberg B, Sundler F. Hypergastrinaemia produces trophic effects in stomach but not in pancreas and intestines.Regul Peptides 1986; 13: 225–233.

    Article  Google Scholar 

  11. Håkanson R, Axelson J, Ekman R, Sundler F. Hypergastrinemia evoked by omeprazole stimulates growth of gastric mucosa but not of pancreas or intestines in hamster, guinea pig and chicken.Regul Peptides 1988; 23: 105–115.

    Article  Google Scholar 

  12. Ryberg B, Axelson R, Håkanson R, Sundler F, Mattsson H. Trophic effects of continuous infusion of [Leu15]- gastrin-17 in the rat.Gastroenterology 1990; 98: 33–38.

    PubMed  CAS  Google Scholar 

  13. Stock-Damgé C, Lhoste E, Aprahamian M, Pousse A. Gastrin modulation of pancreatic growth.Scand J Gastroenterol 1985; 112 (Suppl.): 68–74.

    Article  Google Scholar 

  14. Tatsuta M, Itoh T, Okuda S, Tamura H, Yamamura H. Effect of fundectomy on serum and antral gastrin levels in rats.Gastroenterology 1911; 72: 78–81.

    Google Scholar 

  15. Longnecker DS, Roebuck BD, Yager JD, Lilja HS, Siegmund AB. Pancreatic carcinoma in azaserine-treated rats: induction, classification and dietary modulation of incidence.Cancer 1981; 47: 1562–1572.

    Article  PubMed  CAS  Google Scholar 

  16. Pour PM, Runge RG, Birt D, Gingell R, Lawson T, Nagel D, Wallcave L, Salmasi SZ. Current knowledge of pancreatic carcinogenesis in the hamster and its relevance to the human disease.Cancer 1981; 47: 1573–1587.

    Article  PubMed  CAS  Google Scholar 

  17. Longnecker DS, Jamieson JD, Asch HL. Regulation of growth and differentiation in pancreatic cancer.Pancreas 1989; 4: 256–275.

    Article  Google Scholar 

  18. Labarca C, Paigen K. A simple, rapid and sensitive DNA assay procedure.Anal Biochem 1980; 102: 344–352.

    Article  PubMed  CAS  Google Scholar 

  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent.J Biol Chem 1951; 193: 265–275.

    PubMed  CAS  Google Scholar 

  20. Gasslander T, Chu M, Smeds S, Ihse I. Proliferative response of different exocrine pancreatic cells after surgical pancreaticobiliary diversion in rat.Scand J Gastroenterol 1991; 26: 399–404.

    Article  PubMed  CAS  Google Scholar 

  21. Rehfeld JF, Stadil F, Rubin F. Production and evaluation of antibodies for the radioimmunoassay of gastrin.Scand J Clin Lab Invest 1972; 32: 221–232.

    Article  Google Scholar 

  22. Rehfeld JF. Gastrins in serum.Scand J Gastroenterol 1973; 8: 577–583.

    PubMed  CAS  Google Scholar 

  23. Rehfeld JF. Immunochemical studies on cholecystokinin. I. Development of sequence-specific radioimrnunoassays for porcine triacontatriapeptide cholecystokinin.J Biol Chem 1978; 253: 4016–021.

    PubMed  CAS  Google Scholar 

  24. Byrnes DJ, Henderson L, Borody T, Rehfeld JF. Radioimmunoassay of cholecystokinin in human plasma.Clin Chim Acta 1981; 111: 81–89.

    Article  PubMed  CAS  Google Scholar 

  25. Cantor P. Evaluation of a radioimmunoassay for cholecystokinin in human plasma.Scand J Clin Lab Invest 1986; 46: 213–221.

    Article  PubMed  CAS  Google Scholar 

  26. Pour PM. Cell differentiation during pancreatic carcino- genesis.Scand J Gastroenterol 1988; 23(Suppl 151): 123–130.

    Article  Google Scholar 

  27. Chu M, Borch K, Lilja I, Blomqvist L, Rehfeld JF, Ihse I. Endogenous hypercholecystokininemia model in the hamster, trophic effect on the exocrine pancreas.Pancreas 1992; 7: 220–225.

    Article  PubMed  CAS  Google Scholar 

  28. Edwards BF, Redding TW, Schally AV. The effect of gastrointestinal hormones on the incorporation of tritiated thymidine in the pancreatic adenocarcinoma cell line (WD PaCa).Int J Pancreatol 1989; 5: 191–201.

    PubMed  CAS  Google Scholar 

  29. Valenzuela JE, Walsh JH, Isenberg JI. Effect of gastrin on pancreatic enzyme secretion and gallbladder emptying in man.Gastroenterology 1976; 71: 409–411.

    PubMed  CAS  Google Scholar 

  30. Rehfeld JF. Disturbed islet-cell function related to endog- enous gastrin release: studies on insulin secretion and glucose tolerance in pernicious anemia.J Clin Invest 1976; 58:41–49.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, University Hospital, S-581 85, Linköping, Sweden

    Ming Chu & Kurt Borah

  2. Department of Clinical Chemistry, University of Copenhagen, Rigshospitalet, Denmark

    Jens F. Rehfeld

Authors
  1. Ming Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jens F. Rehfeld
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kurt Borah
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chu, M., Rehfeld, J.F. & Borah, K. Effects of gastric fundectomy and antrectomy on the exocrine pancreas in the hamster. Int J Pancreatol 12, 139–144 (1992). https://doi.org/10.1007/BF02924637

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02924637

Key Words

Search

Navigation