Abstract
A gene for resistance to novobiocin has been self-cloned inH. parainfluenzae, using a high-efficiency plasmid vector pJ1-8: Homospecific and heterospecific transformation using chimeric plasmids containing an insert of thenovr gene ofH. influenzae orH. parainfluenzae has revealed some novel features. There is some evidence from our observations thatH. parainfluenzae also recognises specific uptake sites in the incoming DNA. Transformation with chimeric and non-chimeric plasmids suggests that the homology of the insert to the resident DNA has a major role in the extrachromosomal fixation of chimeric plasmids. It is also inferred that apart from the (passive) internalisation of input DNA from transformasomes, there is also recombination-assisted internalisation.
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Khanolkar, S.M., Notani, N.K. Homospecific and heterospecific transformation with chimeric plasmid DNA inHaemophilus influenzae andHaemophilus parainfluenzae . J. Genet. 65, 31–36 (1986). https://doi.org/10.1007/BF02923533
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DOI: https://doi.org/10.1007/BF02923533