Abstract
Tuber callus by subculture ofDioscorea deltoidea Wall, was grown with different sources of nitrogen on N-free Murashige and Skoog’s basal media supplemented with a high level of kinetin (1, 3, 6, 9 and 12 ml 1-1) and low concentration of auxin (0.01 mg 1-1 2,4-D) for shoot bud differentiation. Shoot and root differentiation was observed only in the case of ammonium nitrate. Other sources of nitrogen failed to produce shoot bud differentiation except in the case of ammonium citrate where tissues were slight green in colour and were recognizable as pro-embryo.
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References
Halperin, W., Wetherell, D. F.: Ammonium requirement for embryogenesisin vitro. - Nature205: 519–520, 1965.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue. - Physiol. Plant.15: 473–496, 1962.
Raghavan, T., Torrey, J. G.: Inorganic nitrogen nutrition of the seedlings of the orchid, Cattleya. - Amer. J. Bot.51 : 264–274, 1964.
Singh, J. N., Singh, J. P.: Effect of increasing levels of nitrate on growth and essential oil content of Japanese mint(Mentha arvensis L. var.piperascens). - J. Sci. Res. B.H.U.23: 87–93, 1972–73.
Yatazawa, M., Furuhashi, K.: Nitrogen sources for the growth of rice callus tissue. - Soil Sci. Plant Nutr.14: 73–84, 1968.
Yatazawa, M., Furuhashi, K., Kurihara, N., Ohnishi, Y.: Amino acid composition of rice callus tissue grown with different kinds of nitrogen sources. - Soil Sci. Plant Nutr.14: 85–88, 1968.
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Singh, J.P. Effect of nitrogen sources on shoot bud differentiation ofDioscorea deltoidea Wall. callus cultures. Biol Plant 20, 436–439 (1978). https://doi.org/10.1007/BF02923347
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DOI: https://doi.org/10.1007/BF02923347