Abstract
Tissue culture was isolated from the stem ofPopulus alba L. ‘pyramidalis’. Callus formation was observed since November till March (1974),i.e. till the formation of calluses suitable for further subeultivation. The most vigorous growth was obtained with the callus culture cultivated on the nutrient medium of DIAZ-COLONet al. (1972) on which more than 11 g of fresh matter was produced after 30 d at the end of the first year of cultivation in darkness, with inoculum weight 1.5-1.8 g. A mild decrease in growth rate of the tissue culture was observed after the first year of cultivation. When illuminated, the originally yellow calluses turned green. The morphological and anatomical structure of the callus culture is also described and cell shape and cell size evaluated.
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References
Bardinskaya, M. S., Moskaleva, V. E.: [Some data on the structure of tracheid cells in callus tissue ofGinkgo biloba L.] In Russ. — Dokl. Akad. Nauk SSSR135: 207–209, 1960.
Brown, C. L., Lawrence, R. H.: Culture of pine callus on denned medium. — Forest Sci.14: 62 to 64, 1968.
Bychenkova, E. A.: The study of proliferation of cambium and parenchyma of branches from trees in culturesin vitro. — Biol. Plant.5: 302–309, 1963.
Chalupa, V.: Control of root and shoot formation and production of trees from poplar callus. — Biol. Plant.16: 316–320, 1974.
Chardenon, M. M. J., Taris, B.: Remarques sur la structure des tissus néoformes et l‘apparition d’organes spécialisés choz quatro cultivars tiePopulus et chez Snlix alba, cultivesin vitro. — Compt, rend. Acad. Sci. (Paris) Sér. D251: 2070–2071, 1960.
Diaz-Colon, J. D., Bowey, R. W., Davis, F. S., Baur, J. R.: Comparative effects and concentration of Picloram, 2,4,5-T and Dicamba in tissue culture. — Physiol. Plant.27: 60–64, 1972.
Durzan, D. J., Campbell, R. A.: Prospects for the mass production of improved stock of forest trees by cell and tissue culture. — Can. J. Forest Res.4: 151–174, 1974.
Erdblský, K.: Wachstumscharakteristik ciner Callus-Gewebenkultur vonPapaver somniferum L. — Acta Fac. Rer. nat. Univ. Comen. Physiol. Plant.3: 1–10, 1971.
Harvey, A. E.: Tissue culture ofPinus monticola on a chemical defined medium. — Can. J. Bot.45: 1783–1785, 1967.
Hricová, D., Strmeň, J., Bauer, Š.: Preparation and characterization of the tissue culture of spruce (Picea excelsa Link). — Biol. Plant,16: 118–122, 1974.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant.15: 473–497, 1962.
Němec, B. et al.: Botanická Mikrotechnika. [Botanical Microtechnics]. — Nakl. ČSAV, Praha 1962.
Street, H. E. (ed.): Tissue Culture and Plant Science. — Acad. Press, London—New York—San Francisco 1974.
Winton, L., Mathes, M. C: Aspen callus. — In: Tissue Cult. Meth. abn. Applt. Pp. 161–165. New York—London 1973.
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Blanáriková, V., Karácsonyi, Š. The isolation of tissue culture ofPopulus alba L. ‘Pyramidalis’ . Biol Plant 20, 14–18 (1978). https://doi.org/10.1007/BF02922924
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DOI: https://doi.org/10.1007/BF02922924