Abstract
Following a 24-h exposure to a solution of polyethylene glycol 4 000 of a —12.66 bar osmotic potential the roots of maize ceased growing. The inhibition of growth was conditioned by the inhibition of cell elongation and division. The elongation of cells was substituted by their radial enlargement which took place both in the peripheral and central root parts. The cells either did not divide at all, or sporadic mitoses still occurred in the roots. The meristematic cells treated were highly vacuolized, chromatin condensation being observed in their nuclei. In contrast to growth processes, differentiation was stimulated: the formation of the secondary wall in protoxylem elements occurred at a shorter distance,i.e. 1 500–2 400 µm from the apex, in comparison with 4000–5 000 µm in the control, this evidently being caused not only by the inhibition of growth, but also by the capacity of cells to differentiate more rapidly. The changes induced by a 24-h exposure to water stress were of a reversible nature; however, a 48-h treatment brought about irreversible changes.
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Acevedo, E., Hsiao, T. C., Henderson, D. W.: Immediate and subsequent growth responses of maize leaves to changes in water status. - Plant Physiol.48: 631–636, 1971.
Burström, H. C., Svensson, S. B.: Hormonal regulation of root development. - In:Kolek, J. (ed.): Structure and Function of Primary Root Tissues. Pp. 121–135. Veda, Bratislava 1974.
Cleland, R.: A dual role of turgor pressure in auxin-induced cell elongation inAvena coleoptiles. - Planta77: 182–191, 1967.
Clowes, F. A. L., Stewart, H. E.: Recovery from dormancy in roots. - Now Phytol.66: 115 to 123, 1967.
Doley, D., Leyton, L.: Effects of growth regulating substances and water potential on the development of secondary xylem inFraxinus. - New Phytol.67: 574–594, 1968.
Gardner, W. R., Nieman, R. H.: Lower limit of water availability to plants. - Science143: 1460 to 1462, 1964.
Gašparíková, O.: Effect of 3-indoleacetic acid on root growth. - Biológia (Bratislava)27: 763 to 769, 1972.
Gonzalez-Bernáldez, F., López-Sáez, J. F., García-Ferrero, G.: Effect of osmotic pressure on root growth, cell cycle and cell elongation. - Protoplasma65: 255–262, 1968.
Gray, L. H., Scholes, M. E.: The effect of ionizing radiation on the broad bean root VIII. Growth rate studies and histological analyses. - Brit. J. Radiol. N. S.24: 176–180, 1951.
Jackson, W. M.: Use of carbowaxes (polyethylene glycols) as osmotic agents. - Plant Physiol.37: 513–519, 1962.
Janes, B. E.: Use of polyethylene glycol as a solvent to increase the osmotic pressure of nutrient solutions in studies on the physiology of water in plants. - Plant Physiol.36: XXIV, 1961.
Janes, B. E.: The effect of molecular size, concentration in nutrient solution, and exposure time on the amount and distribution of polyethylene glycol in pepper plants. - Plant Physiol.54: 226–230, 1974.
Kaufmann, M. R., Eckard, A. N.: Evaluation of water stress control with polyethylene glycol by analysis of guttation. - Plant Physiol.47: 453–456, 1971.
Kirkham, M. B., Gardner, W. R., Gerloff, G. C.: Regulation of cell division and cell enlargement by turgor pressure. - Plant Physiol.49: 961–962, 1972.
Lagerwerff, I. V., Ogata, G., Eagle, H. E.: Control of osmotic pressure of culture solutions with polyethylene glycol. - Science133: 1486–1487, 1961.
Lawlor, D. W.: Plant growth in polyethylene glycol solutions in relation to the osmotic potential of the root medium and leaf water balance. - J. exp. Bot.20: 895–911, 1969.
Lawlor, D. W.: Absorption of polyethylene glycols by plants and their effects on plant growth. - New Phytol.69: 501–513, 1970.
Leshem, B.: Toxic effects of carbowaxes (polyethylene glycols) onFinus halepensis Mill. seedlings. - Plant Soil24: 322–324, 1966.
Leshem, B.: Resting roots ofPinus halepensis: structure, function, and reaction to water stress. - Bot. Gaz.131 (2): 99–104, 1970.
Loescher, W. H., Nevins, D. J.: Turgor-dependent changes inAvena coleoptile cell wall composition. - Plant Physiol.52: 248–251, 1973.
Michel, B. E.: Carbowax 6000 compared with mannitol as a suppressant of cucumber hypocotyl elongation. - Plant Physiol.45: 507–509, 1970.
Torrey, J. G.: The effect of certain metabolic inhibitors on vascular tissue differentiation in isolated pea roots. - Amer. J. Bot.40: 525–533, 1953.
Vartanian, N.: Induction par la sécheresse de racines courtes, tubérisées chez des plantes annuelles: Crucifères et quelques autres familles. - Compt. rend. Acad. Sci. (Paris) Sér. D274: 1497–1500, 1972.
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Čiamporová, M., Luxová, M. The effect of polyethylene glycol-induced water stress on the maize root apex. Biol Plant 18, 173–178 (1976). https://doi.org/10.1007/BF02922796
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DOI: https://doi.org/10.1007/BF02922796