Abstract
The main enzymatic oxidation products of IAA have been tested for biological activity, using as bioassay the straight-growth test ofAvena coleoptile. After being purified by rechromatography, none of these products (including methyleneoxindole) exhibited biological activity within the wide range of concentrations employed; consequently, the results accord with the evidence that IAA is the true hormone of plant growth.
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Andersen, A. S., Moller, I., Hansen, J.: 3-methyleneoxindole and plant growth regulation. - Physiol. Plant.27: 105–108, 1972.
Basu, P. S., Tuli, V.: Auxin activity of 3-methylencoxindole in wheat. - Plant Physiol.50: 499–502, 1972a.
Basu, P. S., Tuli, V.: The binding of indole-3-aoetic acid and 3-methyleneoxindole to plant macromolecules. - Plant Physiol.50: 507–509, 1972b.
Demorest, D. M., Stahmann, M. A.: The binding of the peroxidase oxidation products of indole. 3-acetic acid to histone. - Biochem. biophys. Res. Commun.47: 227 - 233, 1972.
Evans, M. L., Ray, P. M.: Inactivity of 3-methyleneoxindole as mediator of auxin action on cell elongation. - Plant Physiol.52: 186–189, 1972.
Fbenkel, C., Haddon, V. R., Smallheer, J. M.: Promotion of softening and ethylene synthesis in bartlett pears by 3-methyleneoxindole. - Plant Physiol.56: 647 - 649, 1975.
Fukuyama, T. T., Moyed, H. S.: Inhibition of cell growth by photooxidation products of indole- 3-acetic acid. - J. biol. Chem.239: 2392–2397, 1964.
Gaspar, T.H.: Inhibition of root growth as a result of methyleneoxindole formation. - Plant Sci. Lett.1: 115–118, 1973.
Gaspar, TH., Bouchet, M., Fries, D.: The localization of inhibitory oxidation products of indole-3-acetio acid near abscisic acid in theβ-inhibitor complex of lentil root. - Z. Pflanzen physiol.67: 78–85, 1972.
Hinman, R. L., Bauman, C. P.: Reactions of N-bromosuccinimide and indoles. A simple synthesis of 3-bromooxindoles. - J. org. Chem.29: 1206–1215, 1964a.
Hinman, R. L., Bauman, C. P.: Reactions of 3-bromooxindoles. The synthesis of 3-methyleneoxindole.- J. org. Chem.29: 2431–2436, 1964b.
Hinman, R. L., Bauman, C., Lang, J.: The conversion of indole-3-acetic acid to 3-methyleneoxindole in the presence of peroxidase. - Biochem. biophys. Res. Commun.5: 250–254, 1961.
Mendez, J.: Ultraviolet spectral study of indoles. - Microchem. J.15: 1–5, 1970.
Nitsch, J. P., Nitsch, C.: Studies on the growth of coleoptile and first internodo sections. A new sensitive straight-growth test for auxins. - Plant Physiol.31: 94–98, 1956.
Roberts, L. W.: Does 3-methyleneoxindole possess auxin activity? - J. exp. Bot.25: 761–763, 1974.
Tuli, V., Moyed, H. S.: The role of 3-methyleneoxindole in auxin action. - J. biol. Chem.244: 4916–4920, 1969.
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Sabater, F., Cuello, J., Bravo, J.S. et al. Absence of biological activity in oxidation products of indoleacetic acid. Biol Plant 18, 460–463 (1976). https://doi.org/10.1007/BF02922555
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DOI: https://doi.org/10.1007/BF02922555