Abstract
The fungusLentinus tigrinus was cultivated in a synthetic medium. During vegetative growth of mycelium and differentiation of fruiting bodies samples serving for determination of endogenous growth regulators were collected. The material was extracted with methanol, separated by chromatography and the isolated fractions were tested. Substances of auxin type were assayed with coleoptile segments of oat plants, gibberellins by measuring the hypocotyl growth in the lettuce and cytokinins from the degree of chlorophyll decomposition in the leaf segments of barley. Very low content of auxins was found in all phases of growth and differentiation of the fungus. The content of gibberellin substances was relatively high during the whole life of the fungus. The highest content is found in the vegetative mycelium and in the differentiated fruiting body, especially in the cap; it decreases during the differentiation of fruiting bodies. The content of cytokinins is equally high and the pattern of its change resembles that of the gibberellins. Its maximum is reached in young vegetative mycelium, it decreases when fruiting bodies are formed and when they are growing. Minimum is reached in the differentiated stalk, where also the amount of inhibitions is the highest.
Experimental results show that during differentiation of fruiting bodies and their morphogenesis not only gibberellins and cytokinins are of importance, but also substances of an inhibitory character. A relatively high amounts of these substances was secreted into the cultivation medium. In a synthetic nutrient solution they may be easily demonstrated.
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References
Bentley, J. A., Housley, S.: Bioassay of plant growth hormones. - Physiol. Plant.7:405–419, 1954.
Eger, G.: Untersuchungen zur Fruchtkörperbildung des Kulturchampignous. - Mushr. Sci.5: 314–323, 1963.
Frankland, B., Wareing, P. F.: Effect of gibberellic acid on hypocotyl growth of lettuce seedlings. - Nature185: 255–256, 1961.
Gruen, H. E.: Auxin and fungi. - Annu. Rev. Plant Physiol.10: 405–440, 1958.
Kamisaka, S., Yanagishima, N., Masuda, Y.: Effect of auxin and gibberellin on sporulation in yeast. - Physiol. Plant.20: 90–97, 1967.
Kxtbosawa, E.: Trans. Natl. Hist. Soc. Formosa,16: 213–227, 1926. Cited by:Stowe, B. BR., Yamaki Toshio: The history and physiological action of the gibberellins. - Annu. Rev. Plant Physiol.8: 181–216, 1957.
NĚmec, B.: Jak rychle rostou houby? [How quick do the fungi grow]. - Čas. čes. HoubařŠ (Praha)2: 149–152, 1921.
NĚmbc, B.: Dispersion des spores chez quelquesHymenomycetes. - Bull. int. Acad. Sci. Boh.1924: 1–3, 1924.
NĚmeo, B.: Einiges über die Dorsiventralität der Fruchtkörper von Pilzen. - Stud. Plant physiol. Lab. Charles Univ.3: 89–97, 1925.
NĚmec, B.: Wundkompensation und Transplantation bei einigen Polyporazeen. - Bull. int. Acad. Sci. Boh.1935: 8–17, 1935.
NĚmec, B.: Regeneration und Dorsiventralität beiDaedalea. - Bull. int. Acad. Sci. Boh.1936: 167–180, 1936.
NĚmec, B.: Tvar hub. [The shape of fungi.] - Čas. čes. Houbařů (Praha)23: 97–99, 1944.
Osborne, D. J., McCalla, D. R.: Rapid bioassay for kinetin and kinetins using senescing leaf tissue. - Plant Physiol.36: 219–221, 1961.
Park, D.: Evidence for a common fungal growth regulator. - Trans. brit. mycol. Soc.46: 541–548, 1963.
Phinney, B. O., West, Ch. A.: Gibberellins as native growth regulators. - Annu. Rev. Plant Physiol.11: 411–436, 1960.
Pozsáb, B. I.: [Occurence of cytokinins in higher fungi.] In Hungarian. - Mikológiai Közlemänyek2: 84, 1968.
Ray, P. M.: Destruction of auxin. - Annu. Rev. Plant Physiol.9: 81–118, 1958.
Reijndebs, A. F. M.: Les problémes du däveloppement des carpophores des Agaricales et de quelques groupes voisines. - Den Haag 1963.
RypÁČek, V., SladkÝ, Z.: The character of endogenous growth regulators in thE course of development in the fungusLentinus tigrinus. - Mycopathol. Mycol. appl.46: 65–72, 1972.
SladkÝ, Z.: Experimentälná studium květni morfogeneze. [Experimental study of floral morphogenesis.] -Folia Fac. Sci. nat. Univ. Brno12 Biol. 31, Opus8: 1–97, 1971.
Stowe, B. B., Yamaki, T.: The history and physiological action of the gibberellins. - Annu. Rev. Plant Physiol.8: 181–216, 1957.
SzabÓ, L. Gy., Poszsáb, B. I., Kota, M.: Cytokinin activity of the fruiting body ofCoprinus micaceus FR. - Acta Agr. hung.19: 402–403, 1970.
Urayama, T.: Stimulative effect of certain specific bacteria upon mycelial growth and frint formation ofAgaricus bisporus (Lange) sing. - Bot. Mag. (Tokyo)74: 56–59, 1961.
Wessels, J. G. H.: Morphogenesis and biochemical processes inSchizophyllum commune Fb. - Wentia13: 1–113, 1965.
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Dedicated to the 100 anniversary of the birth of professor Němec.
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RypÁek, V., Sladkí, Z. Relation between the level of endogenous growth regulators and the differentiation of the fungusLentinus tigrinus studied in a synthetic medium. Biol Plant 15, 20–26 (1973). https://doi.org/10.1007/BF02922509
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DOI: https://doi.org/10.1007/BF02922509