Abstract
Threadlike particles from mosaic freesia aggregate during and shortly after the purification which is followed by full degradation into insoluble amorphous precipitate. This irreversible process is caused predominantly by host plant proteases. Such degradation can be completely inhibited if the virus complex is purified and stored in the presence of 0.1 mM phenyl-methylsulfonylfluoride. A modified purification method which gives a higher yield and the virua preparation of greater purity is described.
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Abbreviations
- PMSF:
-
phenylmethylsulfonyifluoride
- DIECA:
-
sodium diethyldi. thiocarbamate
- EDTA:
-
disodium ethylenediaminetetraacetate
- PEG-6000:
-
polyethyleneglycol mol. w. 6000
- Tris:
-
tris (hydroxymethyl)aminomethane
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Čech, M., Mokrá, V. & Branišová, H. Stabilization of virus particles from the mosaic diseasedfreesia by phenylmethylsulfonylfluoride during purification and storage. Biol Plant 19, 65–70 (1977). https://doi.org/10.1007/BF02922489
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DOI: https://doi.org/10.1007/BF02922489