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Use of the respiration activity ofThiobacillus ferrooxidans for the specific determination of iron(II, III)

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Abstract

A method is described for the determination of Fe2+ and Fe3+ after its reduction to Fe2+ on the basis of oxygen uptake rate as a function of Fe2+ concentration. By using substratespecificThiobacillus ferrooxidans in combination with the standard addition method a specific determination of iron(II,III) is possible with the determination limit of 3 ámol/L.

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References

  • Drierley C. L.: Bacterial leaching.CRC Crit. Rev. Microbiol.6, 207 (1978).

    Article  Google Scholar 

  • Bin G. A., Suzuki I.: Mechanism of Fe2+ cytochromec reductase ofFerrobacillus ferrooxidaus. Can. J. Biochem.45, 1547 (1967).

    Google Scholar 

  • Din G. A., Suzuki I., Lees H.: Ferrous iron oxidation byFerrobacillus ferrooxidans. Purification and properties of Fe2+ cytochrome reductase.Can. J. Biochem.45, 1523 (1967).

    PubMed  CAS  Google Scholar 

  • Holzbecher Z., Jeník J., Šůcha L., Vláčil F., Vrbský J.:Analytical Chemistry, p. 312. (In Czech) SNTL, Prague 1968.

    Google Scholar 

  • Jílek R., Beranová E., Mandl M.: Isolation and identification of autotrophic thiobacilli. (In Czech) Project report, Veterinary Research Institute, Brno 1981.

    Google Scholar 

  • Koch O. G., Koch-Dedic G. A.:Handbuch der Spurenanalyse, Teil 2. Springer-Verlag, Berlin-Heidelberg-New York 1974.

    Google Scholar 

  • Lacey D. T., Lawson F.: Kinetic of the liquid-phase oxidation of acid ferrous sulfate by the bacteriumThiobacillus ferrooxidans.Biotechnol. Bioeng.12, 29(1970).

    Article  CAS  Google Scholar 

  • Lazaroff N.: Sulfate requirement for iron oxidation byThiobacillus ferrooxidans.J. Bacteriol.85, 78 (1963).

    PubMed  CAS  Google Scholar 

  • Mandl M.: Growth and respiration kinetics ofThiobacillus ferrooxidans limited by CO2 and O2.Biologia (Bratislava)39, 429 (1984).

    CAS  Google Scholar 

  • Margalith P., Silver M., Lundgren D. G.: Sulfur oxidation by the iron bacteriumThiobacillus ferrooxidans.J. Bacteriol.92, 1706 (1966).

    PubMed  CAS  Google Scholar 

  • Shafia F., Wilkinson R. F.: Growth ofFerrobacillus ferrooxidans on organic matter.J. Bacteriol.97, 256 (1969).

    PubMed  CAS  Google Scholar 

  • Silver M.: Oxidation of elementar sulfur and sulfur compounds and CO2 fixation byFerrobacillus ferrooxidans (Thiobacillus ferrooxidans).Can. J. Microbiol.16, 845 (1970).

    PubMed  CAS  Google Scholar 

  • Silver M., Torma A. E.: Oxidation of metal sulfides byThiobacillus ferrooxidans grown on different substrates.Can. J. Microbiol.20, 141 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Silverman M. P., Lundgren D. G.: Studies on the chemoautotrophic iron bacteriumFerrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields.J. Bacteriol.77, 642 (1959).

    Article  PubMed  CAS  Google Scholar 

  • Tabita R., Lundgren D. G.: Utilization of glucose and the effect of organic compounds on the chemolitothrophThiobacillus ferrooxidans.J. Bacteriol.108, 328 (1981).

    Google Scholar 

  • Torma A. E.: The role ofThiobacillus ferrooxidans in hydrometalurgical processes, p. 2 inAdvances in Biochemical Engineering, Vol. 6 (T. K. Ghose, A. Fiechter., N. Blakebrough, Eds.). Springer-Verlag, Berlin-Heidelberg-New York 1977.

    Google Scholar 

  • Tuovinen O. H.: Pathways of the utilization of inorganic sulphur compounds inThiobacillus ferrooxidans, p. 9 inConference Bacterial Leaching, GBF Monograph Series No. 4 (W. Schwartz ed.). Verlag Chemie, Weinheim-New York 1977.

    Google Scholar 

  • Tuovinen O. H., Niemela S. I., Gyllenberg H. G.: Tolerance ofThiobacillus ferrooxidans to some metals.Antoine van Leeuweunnhoek J. Microbiol. Serol.37, 489 (1971).

    Article  CAS  Google Scholar 

  • Tuovinen O. H., Kelly D. P.: Biology ofThiobacillus ferrooxidans in relation to the microbiological leaching of sulphide ores.Z. Allg. Mikrobiol.12, 311 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Tuovinen O. H., Kelly D. P.: Studies on the growth ofThiobacillus ferrooxidans. I. Use of membrane filters and ferrous iron agar to determine viable numbers, and comparison with14CO2-fixation and iron oxidation as measures of growth.Arch. Microbiol.88, 285 (1973).

    CAS  Google Scholar 

  • Tuovinen O. H., Kelly D. P.: Studies on the growth ofThiobacillus ferrooxidans. III. Influence of uranium, other metal ions, and 2:4-dinitrophenol on ferrous iron oxidation and carbon dioxide fixation by cell suspension.Arch. Microbiol.95, 165 (1974a).

    Article  CAS  Google Scholar 

  • Tuovinen O. H., Kelly D. P.: Studies on the growth ofThiobacillus ferrooxidans. V. Factor affecting growth in liquid culture and development of colonies on solid media containing inorganic sulphur compounds.Arch. Microbiol.98, 351 (1974b).

    Article  PubMed  CAS  Google Scholar 

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  1. Veterinary Research Institute, 621 32, Brno, Czechoslovakia

    M. Mandl & H. DoČekalová

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  1. M. Mandl
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  2. H. DoČekalová
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Mandl, M., DoČekalová, H. Use of the respiration activity ofThiobacillus ferrooxidans for the specific determination of iron(II, III). Folia Microbiol 30, 105–109 (1985). https://doi.org/10.1007/BF02922202

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  • DOI: https://doi.org/10.1007/BF02922202

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