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Floral morphogenesis and flowering in aseptic cultures ofBrowallia demissa L

Moríogenese květu a kvetení v aseptických kulturáchBrowallia demissa LINN

Морфогенез цветка и цветение в асептических культурахBrowallia demissa LINN

  • Published:
Biologia Plantarum

Abstract

Floral buds ofBrowallia demissa, at three stages of development, were cultured on Nitsch and Nitsch basal medium. The supplements used include IAA; several cytokinins— benzyladenine, kinetin and 6-benzyl-9 tetrahydropyran-adenine (SD 8339); gibberellic acid (GA3); 2, 3, 5-triiodobenzoic acid (TIBA); arginine and cysteine. All three stages of floral buds failed to complete development. In some treatments stages II and III produced callus and/or roots from the morphological basal end. Cytokinins promoted bud formation whereas both IAA and GA3 depressed bud formation The shoots differentiatedin vitro were capable of setting flowers, fruits and seeds in all the treatments. The seeds were viable. Comparative studies of development of flowersin vivo andin vitro were made. In some treatments the flowers exhibited abnormal corolla, and roecium and gynoecium. Factors affecting normal bud initiation, organization and development are discussed.

Abstract

Květní pupenyBrowallia demissa ve třech vývojových stádiích byly pěstovány na základním mediu Nitsch a Nitsch. Do media byla přidávána IAA, některé cytokininy—benzyladenin, kinetin a 6-benzyl-9-tetrahydropyran-adenin (SD 8339); kyselina giberelová (GA3); kyselina 2,3,5-trijodbenzoová; arginin a cystein. V žádné variantě podle stádia pupenu nedošlo k úplnému vývoji. V některých variantách stádia II a III docházelo ke vzniku kalusu a nebo kořenů z báze pupenů. Cytokininy napomáhaly tvoření pupenů, zatímco jak IAA tak GA potlačily jejich vznik. Stonky diferencovanéin vitro byly schopné vytvořit květy, plody a semena ve všech variantách. Semena byla životaschopná. Byl srovnáván vývoj květůin vitro ain vivo. V některých variantách se v květech vytvářely abnormální květní obaly, androceum a gyneceum. Jsou diskutovány faktory ovlivňjící normální vývoj a organizaci pupene.

Abstract

Выращивались цветочные почкиBrowalia demissa на трех стадиях развития на основной среде по Нитч и Нитч. Добавки к среде: ИУК, некоторые дитокинины—бензиладенин, кинетин и 6-бензил-9-тетрагидропиран-аденин (SD 8339); гибберелловая кислота (GA3); 2,3,5-трийодбензойная кислота; аргинин и цистеин. Полное развитие не происходило ни в одном из вариантов. В некоторых вариантах II и III стадии образовался каллус или корни из основания почек. Цитокинины способствовали образованию почек в то время как ИУК и ГК подавляли их возникновение. Стебли дифференцированныеin vitro были способны образовать цветы, плоды и семена во всех вариантах. Семена были жизнеспособны. Сравнивали развитие цветовin vivo иin vitro. В некоторых вариантах в цветах образовывались аномальные корона, андроцей и гинецей. Обсуждаются факторы влияющие на нормальное развитие и организацию почки.

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  1. Department of Botany, University of Delhi, Delhi 7, India

    P. S. Ganapathy

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Ganapathy, P.S. Floral morphogenesis and flowering in aseptic cultures ofBrowallia demissa L. Biol Plant 11, 165–174 (1969). https://doi.org/10.1007/BF02921735

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