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Microbial conversion of 4-oxoisophorone by thermomonospora curvata using an air-bubbling hollow fiber reactor

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Abstract

Microbial conversion of 4-oxoisophorone (OIP) by thermophilic bacteriumThermomonospora curvata was attempted in a continuous process.

The correlation between cell growth and microbial conversion was first examined in a batch culture. The results indicated that this microbial conversion was strongly dependent upon cell growth. In a continuous microbial conversion of OIP using a continuous stirred tank reactor, the cell density in the reactor seemed to be the limiting factor in the OIP conversion. Therefore, we developed an air-bubbling hollow fiber reactor to achieve a high density culture. By using this bioreactor, more than 3.3 times higher productivity was achieved. In addition, during the process, only a slight cell contamination to the product was observed. Therefore, this bioreactor is suitable for the continuous microbial conversion, considering further downstream processes and high productivity.

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Authors and Affiliations

  1. Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, 153, Meguro-ku, Tokyo

    Koji Sode & Isao Kargbe

  2. Research Laboratory of Resources Utilization, Tokyo, Institute of Technology, 4259, Nagatsuta-cho, 227, Midori-ku, Yokohama

    Isao Kargbe

  3. Central Research Institute, Japan Tobacco Inc., 6-2 Umegaoka, 227, Midori-ku, Yokohama

    Minoru Honda & Yoichi Mikami

  4. Research Department, Engineering Division, NOK Corp., 4-3-1 Tsujido- shinmachi, Fujisawa, 251, Japan

    Tsuyoshi Yanagimoto

Authors
  1. Koji Sode
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  2. Minoru Honda
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  3. Yoichi Mikami
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  4. Tsuyoshi Yanagimoto
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  5. Isao Kargbe
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Sode, K., Honda, M., Mikami, Y. et al. Microbial conversion of 4-oxoisophorone by thermomonospora curvata using an air-bubbling hollow fiber reactor. Appl Biochem Biotechnol 19, 209–220 (1988). https://doi.org/10.1007/BF02921484

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  • DOI: https://doi.org/10.1007/BF02921484

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