Abstract
Batch culture experiments of three different strains ofSaccharomyces cerevisiae have been carried out. The first strain was transformed by a plasmid pCYG4, which carries the glutamate dehydrogenase (NADP-GDH, E.C. 1.4.14) gene conferring an 11-fold increase in activity. The second was transformed by the same plasmid, but without NADP-GDH, and the third was the wild type. The specific growth rates of the two recombinant DNA strains were below that of the wild type, which can be related to extra plasmid protein production.
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Vichido, I., Mora, Y., Quint, C., Palacios, R., and Mora, J. (1978),J. Gen. Microbiol. 106, 251–259.
Bogonez, E., Satrastegui, J., and Machado, A. (1985),J. Gen. Microbiol. 131, 1425–1432.
Magasaniz, B., Privai, M. J., Brenchley, J. E., Tyler, B. M., De Leo, A. B., Streicher, S. L., Bender, R. A., and Paris, C. G. (1974),Current Topics in Cellular Regulation.8, 119–138.
Meers, J. L. and Tempest, D. W. (1970),J. Gen. Microbiol. 64, 187–194.
Tempest, D. W., Meers, J. L., and Brown, C. M. (1970),Biochemical J. 117, 405–407.
Burn, V. J., Turner, P. R., and Brown, C. M. (1974),Antonie van Leeuwenhoek 40, 93–102.
Tempest, D. W., Meers, J. L., and Brown, C. M. (1973),The Enzymes of Glutamine Metabolism (Prusiner, S. and Stadtman, E. R. eds.), Academic, New York, 167–182.
Nagasu, T. and Hall, B. D. (1985),Gene 37, 247–253.
Moye, S. W., Amuro, N., Rao, J. K. M., and Zalkin, H. (1985),J. Biol. Chem. 14, 8502–8508.
Lima Filho, J. L. and Ledingham, W. M. (1987),Proc. 4th European Congress on Biotechnology, Amsterdam 3, 82.
Lima Filho, J. L. and Ledingham, W. M. (1987),Biotechnology Techniques 1, 145–150.
Lima Filho, J. L. (1987), Ph.D. Thesis, University of St. Andrews, Scotland.
Bergmeyer, H. U. (1974),Methods in Enzymatic Analysis, 2nd ed., vols. III and IV, Academic, New York.
Lee, G. M., Song, K. B., Rhee, S. K., and Han, M. H. (1986),Biotechnol. Lett. 6, 385–390.
Helling, R. B., Kinney, T., and Adams, J. (1981),J. Gen. Microbiol. 123, 129–141.
Old and Primrose (1985),Principles of Gene Manipulation, Blackwell, London.
Srienc, F., Campbell, J. L., and Bailey, J. E. (1986),Biotechnol. Bioeng. 28, 996–1006.
Burn, V. J., Turner, P. R., and Brown, M. (1974),Antonie van Leeuwenhoek 40, 93–102.
Caulcott, C. A. (1984),Biochemical Society Transactions, 1140–1141.
Grenson, M., Dubois, E., and Piotrowska, M. (1974),Molec. Gen. Genet. 128, 73–85.
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Filho, J.L.L., Ledingham, W.M. Studies onSaccharomyces cerevisiae arrying the plasmid pCYG4 related with ammonia assimilation. Appl Biochem Biotechnol 19, 27–32 (1988). https://doi.org/10.1007/BF02921463
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DOI: https://doi.org/10.1007/BF02921463