Skip to main content
SpringerLink
Log in

Stability and recovery ofPenicillium funiculosum cellulase under use conditions

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The stability ofPenicillium funiculosum cellulase has been investigated under the conditions used for cellulose hydrolysis. Fifty five percent of filter paper activity (FPA) was inactivated on incubation at 50°C for 24 h, whereas there was no loss in endoglucanase and β-glucosidase activity. The addition of 2% polyethylene glycol (PEG) during incubation stabilized the FPA. The influence of pH during fermentation on the thermal stability of the enzyme is discussed. The recovery of enzymes after hydrolysis of bagasse at 50°C was between 8 and 14%. Under the optimal conditions of elution, the recovery of enzyme was 35% (1). Increasing the enzyme to the substrate ratio fivefold and presence of PEG during hydrolysis resulted in 80, 83, and 95% recovery of β-glucosidase, FPA, and endoglucanase activity, respectively. Index Entries: Stability; recovery of cellulase P.funiculosum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rao, M., Seeta, R., and Deshpande, V. (1983),Biotechnol. Bioeng. 25, 1863.

    Article  CAS  Google Scholar 

  2. Mandels, M. and Weber, J. (1969),Adv. Chem. Ser. 95, 391.

    Article  CAS  Google Scholar 

  3. Wood, T. M. (1975),Biotechnol. Bioeng. Symp. 5, 111.

    CAS  Google Scholar 

  4. Bastavade, Deshpande, V. V., Joglekar, A. V., Lakshmi Kantham, B. C., Mishra, C. M., Phansalkar, S. Rao, M., Seeta, R., Srinvasan, M. C., and Jagannathan, V. (1977),Cellulolytic enzymes of a Penicillium strain in Proc. Bioconversion Symp., Delhi, I. I. I. and Ghose, T. K., eds., p. 143.

  5. Streamer, M., Eriksson, K. E., and Pettersson, B. (1975),Eur. J. Biochem. 59, 607.

    Article  CAS  Google Scholar 

  6. Mishra, C. M., Rao, M., Seeta, R., Srinivasan, M., and Deshpande, V. V. (1984),Biotechnol. Bioeng. 16, 370–73.

    Article  Google Scholar 

  7. Mandels, M., Medeiros, J. E., Andreotti, R. E., and Bissett, P. H. (1981),Biotechnol. Bioeng. 23, 2009.

    Article  CAS  Google Scholar 

  8. Reese, E. T. (1980),J. Appl. Biochem. 2, 36.

    CAS  Google Scholar 

  9. Reese, E. T. and Mandels, M. (1980),Biotechnol. Bioen. 23, 2009.

    Google Scholar 

  10. Durand, H., Soucaille, P., and Tiraby, G. (1984),Enz. Microb. Technol. 6, 175.

    Article  CAS  Google Scholar 

  11. Karube, I., Tanaka, S., and Shirai, T. (1977),Biotechnol. Bioeng. 19, 1183.

    Article  CAS  Google Scholar 

  12. Mishra, C. M., Deshpande, V., and Rao, M.,Enz. Microb. Technol. 5, 342.

  13. Rye, D. Y. and Mandels, M. (1980),Enz. Microb. Technol. 2, 91.

    Article  Google Scholar 

  14. Ghose, T. K. and Bisaria, V. S. (1979),Biotechnol. Bioeng. 21, 131.

    Article  CAS  Google Scholar 

  15. Castanon M. and Wilke, C. R. (1981),Biotechnol. Bioeng. 23, 1365.

    Article  CAS  Google Scholar 

  16. Berghem, L. E. R. and Pettersson, L. G. (1973),Eur. J. Biochem. 37, 21.

    Article  CAS  Google Scholar 

  17. Lowry, O. H., Rosebrough, N. J., Farr, A. J., and Randall, R. J. (1951),J. Biol. Chem. 193, 265.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, 411008, Poona, India

    Vasanti Deshpande, R. Seeta, Chitra Mishra & Mala Rao

Authors
  1. Vasanti Deshpande
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Seeta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chitra Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mala Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

NCL Communication Mo. 4079.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Deshpande, V., Seeta, R., Mishra, C. et al. Stability and recovery ofPenicillium funiculosum cellulase under use conditions. Appl Biochem Biotechnol 19, 11–19 (1988). https://doi.org/10.1007/BF02921461

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02921461

Keywords

Search

Navigation