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Gasometric method of water deficit measurement in leaves

Gazometrická metoda ke stanovení vodního defieitu v listech

Газометрический метод измерения водного дефицита в листьях

  • Published:
Biologia Plantarum

Abstract

A gasometric method was developed for measuring water deficit in leaves. For a leaf at full turgor the amount of water penetrating into the tissue after removing the air from intercellular spaces by means of a vacuum pump, is equal in volume to the gas removed from the intercellular spaces. In a leaf with a water deficit the amount of the infiltrating water is greater than the removed gas volume by the amount egual to the water deficit. Determination of the volumes of the gas removed and penetrating water enables water deficit, if any, to be calculated. Comparative measurements carried out on five plant species confirmed the correctness of the method suggested. Reduction of the measuring time allowed to eliminate completely the sources of errors associated with the growth of tissue and loss of dry weight during respiration.

Abstract

V práci je popsána nová gazometrická metoda ke stanovení vodního deficitu v listech. Prindipem metody je stanovení objemu vzduchu vysátého vývěvou z mezibuněěných prostor sledovaných listů. U plně turgescentního listu se množství vody vniklé do pletiva při podtlaku rovná objemu plynu vysátého z intercelulár. U listu majícího vodní deficit je množství vniklé vody větší než objem plynu z intercelulár o množství odpovídající vodnímu deficitu. Váhové stanovení vniklé vody a současné stanovení objemu plynu z mezibuněčných prostor tedy umožňuje výpočet vodního deficitu. Srovnávací měření na pěti druzích rostlin potvrdila správnost navržené metody. Zkrácením doby potřebné k měření se zcela vyloučily zdroje chyb spojené a růstem pletiva a prodýcháním sušiny během měření.

Abstract

Разработан метод измерения водного дефицита в листьях. Измерительное устройство работает на принцнне извлечения газа из межклетников вакуум-насосом и измерения его обьема. При полном тургоре количество воды поступившей в ткань равно по объему газу извлеченному из межклетникоВ. В условиях водного дефицита объем воды больший чем объем газа на величину равную водному дефициту. По измерении объема воды и газа можно расчитать водный дефицит. Сравнительные измерения на пяти видах растений дали хорошие результаты оправдывающие применение предлагаемого метода. Уменьшение длительности измерения исключило ошибки связанные с ростом ткани и потерей сухого веса при дыхании.

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Authors and Affiliations

  1. Department of Plant Physiology, The University of Warsaw, Poland

    J. Czerski

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  1. J. Czerski
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Address: Krakowskie Przedmieśeie 26/28, Warszawa 64, Poland.

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Czerski, J. Gasometric method of water deficit measurement in leaves. Biol Plant 10, 275–283 (1968). https://doi.org/10.1007/BF02921010

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  • DOI: https://doi.org/10.1007/BF02921010

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