Abstract
During the 24 hours’ washing of14C MNU and14C MMS-treated seeds the amount of DNA 7-methylguanine as well as the total alkylation of DNA, RNA and proteins in cells of the embryo was enhanced. The drying of shortly washed14C MNU-treated seeds to 30% and 15% water content led to, a similar increase of alkylation. In accordance with the changes in the biological damage, the amount of single strand breaks and/or alkali labile sites in DNA dropped down during a 19 h washing period of MNU-treated seeds but changed only slightly in MMS-treated and 19 hours washed seeds. The drying of 0·5 or 18 h resp. 19 h washed seeds treated either with MNU or MMS to 15 per cent water content increased the amount of single strand breaks and/or alkali labile sites in DNA.
Abstract
V pr⫲běhu dvacetičtyřhodinového vypírání semen ječmene ovlivněných14C MNU a14C MMS, se zvýšila celková alkylace DNA, RNA a protein⫲ a množství DNA 7-methylguaninu v buňkách embryí. Podobné zvýšení alkylace bylo zjištěno i po vysušení semen ovlivněných14C MNU a krátce promytých vodou. Ve shodě se změnami ve stupni biologických účink⫲ obou mutagen⫲ se snížilo množství jednořetězcových zlom⫲ v DNA resp. míst na DNA labilních k alkaliím pouze po p⫲sobení MNU a 19 h vypírání semen, zatímco po p⫲sobení MMS a stejné době vypíráni nebyly nalezeny podstatné změny v tomto směru. Vysoušení semen ovlivněných MNU a MMS, at již krátce nebo dlouze promývaných, vedlo vždy ke zvýšení množství jednořetězcových zlom⫲ resp. míst labilních k alkaliím na DNA.
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References
Bender, K., Gaul, H.: Nachwäsche, Rücktrocknung und Lagerung bei ÄMS—behandelten Gerstensamen.—Radiat. Bot.6: 505–518, 1966.
Brunner, H., Mikaelsen, K.: Beeinflussende Faktoren in der mutagenen Wirkung von Äthylmethansulfonat auf Gerste.—Z. Pflanzenzüchtung.66: 9–36, 1971.
Brunner, H., Mikaelsen, K., Nagl, K., Konzak, C. F.: Methodische Untersuchungen der beeinflussender Faktoren in der Diäthylsulfat-Mutagenwirkung bei Gerste.—Bodenkultur19: 336–345, 1968.
Gichner, T., Gaul, H., Omura, T.: The influence of post-treatment washing and redrying of barley seeds on the mutagenic activity of N-methyl-N-nitroso-urea and N-ethyl-N-nitrosourea.— Radiat. Bot.8: 499–507, 1968.
Gichner, T., Velemínský, J., Pokorný, V., Švachulová, J.: Influence of post-treatment washing on the mutagenic effects in propyl methanesulfonate—and isopropyl methanesulfonate— treated barley seeds.—Radiat. Bot.12: 221–227, 1972.
Khalathar, A. S., Bhatia, C. R.: Uptake of C14-methyl methanesulfonate by wheat embryos.— In: Proc. Symp. Radiation and Radiomimetic subst. in Mut. Breeding, Pp. 48–59. Bombay, India 1970.
Konzak, C. F., Nilan, R. A., Froese-Gertzen, E. E., Foster, R. J.: Factors affecting the biological action of mutagens.—In: Induction of Mutations and the Mutation Process. Pp. 123–132. Publ. House Czechoslov. Acad. Sci., Praha 1965.
Konzak, C. F., Wickham, I. M., DeKock, M. J.: Advances in methods of mutagen treatment. In: Induced Mutations and Plant Improvement. Pp. 95–119, IAEA, Vienna 1972.
Mikaelsen, K., Brunner, H., Li, W. C.: Influence of postwash time on the mutagenic effects of ethylmethanesulphonate (EMS) in barley seeds.—Hereditas69: 15–18, 1971.
Narayanan, K. R., Konzak, C. F.: Influence of chemical posttreatments on the mutagenic officiency of alkylating agents. In: Induced Mutations in Plants. Pp. 281–304.—IAEA, Vienna 1969.
Osone, K., Mikaelsen, K.: Studies on embryo development and changes in sensitivity to gamma radiation and alkylating agents (MMS and EMS) in rice seeds during soaking in water.—In: Rice Breeding with induced Mutations III. Pp. 103–115.—IAEA, Vienna 1971.
Osterman-Golkar, S., Ehrenberg, L., Wachtmeister, C. A.: Reaction kinetics and biological action in barley in mono-functional methane sulfonic esters.—Radiat. Bot.10: 303–327, 1970.
Strauss, B., Coyle, M., Robbins, M.: Consequence of alkylation for the behaviour of DNA.— Ann. N. Y. Acad. Sci.163/2: 765–787, 1969.
Velemínský, J., Osterman-Golkar, S., Ehrenberg, L.: Reaction rates and biological action of N-methyl- and N-ethyl-N-nitrosourea.—Mutation Res.10: 169–174, 1970.
Velemínský, J., Zadražil, S., Gichner, T.: Repair of single strand breaks in DNA and recovery of induced mutagenic effects during the storage of ethyl methanesulphonate-treated barley seeds.—Mutations Res.14: 259–261, 1972.
Velemínský J., Zadražil, S., Pokorný, V., Gichner, T., Švachulová, J.: Repair of single strand breaks and fate of N-7-methylguanine in DNA during the recovery from genetical damage induced by N-methyl-N-nitrosourea in barley seeds.—Mutation Res.17: 49–58, 1973a.
Velemínský, J., Zadražil, S., Pokorný, V., Gichner, T., Švachulová, J.: Storage-effect in barley. Changes in the amount of DNA lesions induced by methyl- and ethyl methanesulphonate. —Mutation Res.19: 73–81, 1973.
Zadražil, S., Velemínský, J., Pokorný, V., Gichner, T.: Repair of DNA lesions in barley induced by monofunctional alkylating agents.—Studia biophysica.36/37: 271–276, 1973.
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Zadražil, S., Pokorný, V., Velemínský, J. et al. Changes in the DNA lesions induced by alkylating agents during the post-treatment washing and redrying of barley seeds. Biol Plant 16, 7–13 (1974). https://doi.org/10.1007/BF02920814
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DOI: https://doi.org/10.1007/BF02920814