Abstract
The components of synthesis gas, CO, H2, and CO2, may be converted into CH4 biologically through either acetate or H2/CO2 as intermediates. Of these two routes, conversion through H2/CO2 is preferred. This paper presents results of mixed-culture studies employing the photosynthetic bacteriumR. rubrum for converting CO to CO2 and H2 by the water gas shift reaction and two methanogens,M. formicicum andM. barkeri, for converting CO2 and H2 into CH4. Results are presented for triculture operation in two types of reactors, the packed bubble column and the trickle-bed reactor.
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McCullough, G. R., Roberts, S. C., and van der Burgt, M. J. (1982),Energy Prog. 2, 69.
Koh, W. K. (1982),Hydro. Proc. 61(6), 85.
Eagen, C. A., Jr., and Wessenhott, R. D. (1981),Energy. Prog. 1, 104.
Kaplan, L. J. (1982),Chem. Engr. 89(6), 64.
Richardson, J. T. (1973),Hydro. Proc. 52(12), 99.
Jockei, H. and Triebskorn, B. E. (1973),Hydro. Proc. 52(1), 93.
Courty, D. and Chaumette, P. (1987), Energy Prog.7, 23.
Barik, S., Corder, R. E., Clausen, E. C., and Gaddy, J. L. (1987),Energy Prog. 7, 157.
Vega, J. L., Klasson, K. T., Kimmel, D. E., Ko, C. W., Clausen, E. C., and Gaddy, J. L. (1989), “Advanced Studies of the Biological Conversion of Coal Synthesis Gas to Methane. Topical Report on Task 4. Sulfur Toxicity,” US Department of Energy, Morgantown Energy Technology Center, Contract No. DE-AC-21-86MC23281.
Brown, L. M. (1987), MS thesis, University of Arkansas, Fayetteville, AR.
Buchanan, R. E. and Gibbons, N. E., eds. (1974),Bergey’s Manual of Determinative Microbiology, 8th ed., Williams and Wilkins, Baltimore, MD. 12. Kluyver, A. J. and Schnellen, C. G. (1947),Arch. Biochem. 14, 57.
Elmore, B. B., Vega, J. L., Ackerson, M. D., Clausen, E. C., and Gaddy, J. L. (1988), “Bioconversion of Synthesis Gas Into Ethanol or Methane,” presented at the IGT Symposium on Gas, Oil and Coal Biotechnology, New Orleans, LA.
Bailey, J. E. and Ollis, D. F. (1986),Biochemical Engineering Fundamentals, 2nd ed., McGraw-Hill, New York.
Antorrena, G. A. (1987), Department of Chemical Engineering, University of Arkansas, Fayetteville, AR.
McInerney, M. J., Bryant, M. P., and Pfennig, N. (1979),Arch. Microbiol. 122, 129.
Genthner, B. R. S., Davis, M. P., and Bryant, M. P. (1981),Appl. Environ. Microbiol. 42, 12.
Ko, C. W., Vega, J.L., Clausen, E. C., and Gaddy, J. L. (1989), “Utilization of CO, CO2 and H2 in Synthesis Gas by a Co-culture ofPeptostreptococcus productus andMethanobacterium formicicum through Interspecies H2 Transfer”, submitted toBiotechnol. Bioengr.
Charpentier, J. C. (1981),Advances in Chemical Engineering, vol.11, Academic, New York.
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Klasson, K.T., Cowger, J.P., Ko, C.W. et al. Methane production from synthesis gas using a mixed culture ofR. rubrum M. barkeri, and M. formicicum . Appl Biochem Biotechnol 24, 317–328 (1990). https://doi.org/10.1007/BF02920256
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DOI: https://doi.org/10.1007/BF02920256