Summary
Three human T cell clones (TCC) specific for purified protein derivative of Mycobacterium tuberculosis were incubated in the presence of polybrene and phytohemagglutinin with irradiated mononuclear cells from one individual exhibiting seropositivity for human immunodeficiency virus (HIV) and high levels of circulating p24 antigen. After three weeks, TCC showed HIV integration in their DNA, as shown by polymerase chain reaction analysis and Southern blot technique. All the three HIV-infected TCC maintained their ability to recognize the specific antigen, even if their proliferative ability was reduced. The ability of the HIV-infected TCC to produce IL-2, IL-4 and IFN-y in response to phorbol myristate acetate plus anti-CD3 monoclonal antibody was decreased, whereas their ability to produce TNF-α was unaffected or even enhanced. Two our. of the three HIV-infected TCC showed the ability to provide helper function for polyclonal immunoglobulin production when cocukured with autologous B cells in the absence of any stimulant. These data suggest that in vitro infection of normal human TCC may provide a useful model for the study of immunological alterations induced by HIV.
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References
Edelman A. S., Zolla-Pazner S.: AIDS: a syndrome of immune dysregulation, dysfunction and deficiency — FASEB J.3, 14, 1989.
Fauci A. S.: The human immunodeficiency virus: infectivity and mechanisms of pathogenesis — Science239, 617, 1988.
Folks T. M., Clouse K. A., Justement J., RabsoN A., Duh E., Kehrl J. H., Fauci A. S.: Tumor necrosis factor-alpha induces the expression of the human immunodeficiency virus from a chronically infected T cell clone — Proc. nat. Acad. Sci. (Wash.)86, 2365, 1989.
Maggi E., Del Prete G. F., Macchia D., Parroxchi P., Tiri A., Chrétien I., Ricci M., Roma gNaNi, S.: Profiles of lymphokine activities and helper function for IgE in human T cell clones — Europ. J. Immunol.18, 1045, 1988.
Maggi E., Macchia D., Parronchi P., Mazzetti M., RavIna A., Milo D., Romagnani S.: Reduced production of interleukin-2 and interferon-γ and enhanced helper activity for IgG synthesis by cloned CD4+ T cells from patients with AIDS — Europ. J. Immunol.17, 1685, 1987.
Meuer S. C., Hussey R. E., Caxtrell D. A., Hodgson J. C., Schlossman S. F., Smith K. A., Reinherz E. L.: Triggering of the T3-Ti antigen-receptor complex results in clonal T-cell proliferative through an interleukin-2-dependent autocrine pathway — Proc. nat. Acad. Sci. (Wash.)81, 1509, 1984.
Romagnani S.: Cytokines involved in activation, growth and differentiation of human B cells — J. immunol. Res.1, 41, 1989.
Sambrook J., Fritsch E. F., Maniatis T. (Eds): Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989; 2nd ed., vol. 1, chapt. 7.
Spits H., Yssel H., Takebe Y., Arai N., Yokota T., Lee F., Arai K. I., Baxchereau J., De Vries J.: Recombinant interleukin-4 promotes the growth of human T cells — J. Immunol.139, 1142, 1987.
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This work was supported by grants from theConsiglio Nazionale delle Ricerche (CNR), theMinistero della Sanità, Istituto Superiore di Sanità (contract AI-25372), and theAssociazione Italiana per la Ricerca sul Cancro (AIRC), Roma, Italy. P. Biswas, C. Simonelli and M. Mazzetti were supported by a fellowship of AIRC. M.-P. Piccinni was supported by a fellowship of European Economic Community.
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Macchia, D., Simonelli, C., Parronchi, P. et al. In vitro Infection with hiv of antigen-specific t cell clones derived from hiv-seronegative individuals. effects on cytokine production and helper function. La Ricerca Clin. Lab. 21, 85–90 (1991). https://doi.org/10.1007/BF02919116
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DOI: https://doi.org/10.1007/BF02919116