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Increased expression of complement receptor type 1 (CR1, CD35) on human peripheral blood T lymphocytes after polyclonal activation in vitro

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Abstract

The receptor for C3b and C4b—complement receptor type 1 (CR1, CD35)—is present on a variety of cell types including erythrocytes, phagocytic cells, B lymphocytes and a small sub-population of T lymphocytes. The function of the receptor varies according to the different cell types, but a T lymphocytes the function is as yet not known. The present study concerns the influence of polyclonal stimulation on CR1-expressing T lymphocytes. Incubation with PHA resulted in a dose-dependent increase in the number of CR1-positive T lymphocytes. The CR1-expression T lymphocytes were found in both the CD4- and the CD8-positive subpopulation, but a significant stimulatory increase was only found in the CD4-positive population. A significant increase in the number of CR1-expressing T lymphocytes was found when monocytes were present during stimulation, indicating an importance of monocytes and/or monocyte products. However, the increase was not regulated by arachidonic acid metabolites of the cyclo-oxygenase pathway as indomethacin failed to inhibit the increase. Neither did rIL-Iα, rIL-1β, rTNFα nor rIL-6 alter the number of CR1-expressing T lymphocytes. The results of this study indicate a role for CR1 on T lymphocytes in the regulation of the immune system.

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Authors and Affiliations

  1. Laboratory of Medical Immunology, State University Hospital, Copenhagen, Denmark

    Anne Rødgaard, Gunnar Bendixen & Klaus Bendtzen

  2. Medical Department TTA, Rigshospitalet, State University Hospital, Copenhagen, Denmark

    Gunnar Bendixen & Klaus Bendtzen

  3. Department of Rheumatology, Hvidovre Hospital, University Hospital, Kettgards Alle 30, DK-2650, Hvidovre, Denmark

    Anne Rødgaard

  4. Department of Rheumatology, Herlev Hospital, Copenhagen, Denmark

    Svalgaard Thomsen

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  1. Anne Rødgaard
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  2. Svalgaard Thomsen
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  3. Gunnar Bendixen
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  4. Klaus Bendtzen
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Rødgaard, A., Thomsen, S., Bendixen, G. et al. Increased expression of complement receptor type 1 (CR1, CD35) on human peripheral blood T lymphocytes after polyclonal activation in vitro. Immunol Res 14, 69–76 (1995). https://doi.org/10.1007/BF02918498

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