Abstract
To determine which of a variety of inorganic and organic selenium compounds could best stimulate glutathione peroxidase, human lymphocytes were cultured with a number of selenium sources. The phytohemagglutinin-transformed lymphocytes were cultured in the presence of75Se bound to serum proteins (25% v/v) or 10−7 M concentrations of [75Se]-selenite, [75Se]-selenate, [75Se]-selenocystine, and [75Se]-selenomethionine. Organic forms of selenium were taken up in preference to inorganic forms. Control cultures, from which exogenous selenium had been omitted, showed a decreased level of glutathione peroxidase activity at the end of a 4 d culture period. Of the Se sources tested, [75Se]-selenocystine and [75Se]-labeled fetal calf serum proteins increased enzyme activity significantly, 79 and 47%, respectively, but selenite increased activity only by 7%. These results indicate that selenium from the two organic sources is most readily available for glutathione peroxidase synthesis.
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Karle, J.A., Kull, F.J. & Shrift, A. Uptake of selenium-75 by PHA-stimulated lymphocytes. Biol Trace Elem Res 5, 17–24 (1983). https://doi.org/10.1007/BF02916923
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DOI: https://doi.org/10.1007/BF02916923