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The use of Soybean Agglutinin (SBA) for Bone Marrow (BM) purging and hematopoietic progenitor cell enrichment in clinical bone-marrow transplantation

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Abstract

Soybean agglutin (SBA) is a plant lectin that has been used to fractionate bone marrow cells. It binds bone marrow mononuclear cells, including mature myeloid, erythroid, and lymphoid cells, but has very low binding affinity and no toxic effect to the human hematopoietic cells. In this article we describe the possibilities of enriching bone-marrow-derived CD34 + hematopoietic progenitor cells using SBA. As the method is simple and elegant, SBA is of vast importance to the field of clinical bone marrow transplantation. Index Entries: Soybean agglutinin; hematopoietic progenitor cell; bone marrow transplantation.

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  1. Bone Marrow Transplantation Department, Hadassah University Hospital, 91120, Jerusalem, Israel

    Arnon Nagler

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  1. Arnon Nagler
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  2. Shoshana Morecki
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  3. Shimon Slavin
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Correspondence to Arnon Nagler.

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Arnon, N., Shoshana, M. & Shimon, S. The use of Soybean Agglutinin (SBA) for Bone Marrow (BM) purging and hematopoietic progenitor cell enrichment in clinical bone-marrow transplantation. Mol Biotechnol 11, 181–194 (1999). https://doi.org/10.1007/BF02915811

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