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Purification of hematopoietic stem cells from bone marrow

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Abstract

Lifelong production of blood cells is sustained by hematopoietic stem cells (HSC) which reside at a very low frequency within the bone marrow (BM). The use of flow cytometry has been critical in establishing methods to isolate and characterize HSCs and their progenitors. For more than 30 years, researchers have uncovered many novel markers that when used in combination significantly enhance the purification of HSCs from murine and human bone marrow. Here, we review the phenotypic markers and strategies used to purify HSCs.

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Acknowledgments

This work was supported by grants 31301161, 81270603, and 81570201 from the National Natural Science Foundation of China (NSFC).

Authors’ contributions

C.T. drafted the paper. Y.Y. critically revised the paper. Y.Z. acquired and analyzed the data and critically revised the paper. All authors approved all versions including the final version and are responsible for the accuracy and integrity of all aspects of the manuscript.

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Authors and Affiliations

  1. National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, People’s Republic of China

    Chen Tian & Yizhuo Zhang

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  1. Chen Tian
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  2. Yizhuo Zhang
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Correspondence to Yizhuo Zhang.

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The authors declare that they have no conflicts of interest.

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Tian, C., Zhang, Y. Purification of hematopoietic stem cells from bone marrow. Ann Hematol 95, 543–547 (2016). https://doi.org/10.1007/s00277-016-2608-z

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  • DOI: https://doi.org/10.1007/s00277-016-2608-z

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