Abstract
The induction of sister chromatid exchanges (SCEs) inVicia faba root-tip cells after short-term (2 h) and long-term (24 h) treatments with alkylating agents (N-methyl-N-nitrosourea, ethyl methanesulphonate) and maleic hydrazide was studied. The primary roots were treated with mutagens before or after 5-bromodeoxyuridine (BrdU) incorporation into DNA and the influence of mutagen application on SCE induction in the cells with non- and BrdU-substituted chromosomal DNA. On the contrary, application of maleic hydrazide after the incorporation of BrdU into DNA strongly increased the rate of SCEs. The lowest limit concentrations of mutagens capable of significantly increasing SCE frequency in the cells with non-substituted DNA after the long-term treatment were estimated.
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Abbreviations
- BrdU:
-
5-bromodeoxyuridine
- dThd:
-
deoxythymidine
- EMS:
-
ethyl methanesulphonate
- FdU:
-
5-fluorodeoxyuridine
- MH:
-
maleic hydrazide
- MNU:
-
N-methyl-N-nitrosourea
- SCE:
-
sister chromatid exchange
- Urd:
-
uridine
References
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Veselská, R., Kuglík, P. & Relichová, J. The influence of incorporated bromodeoxyuridine on mutagenicity testing by sister chromatid exchange induction inVicia faba root tip cells. Biol Plant 37, 9–14 (1995). https://doi.org/10.1007/BF02912991
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DOI: https://doi.org/10.1007/BF02912991