Abstract
A novel electrophoresis technique, in which a strip of perflurosulfonic-acid (nafion 117) membrane was used to replace the conventional separation column and liquid buffer solution within, was developed and employed to separate the mixture of dopamine and epinephrine under a low separation voltage of 100 V with quadruple pulses amperometry detection. It was showed that the so-called Nafion membrane electrophoresis could be one of very simple and easy method and has the potentiality to be used to separate and analyze some small organic biologic molecules.
Similar content being viewed by others
References
Knox J H, Grant I H. Miniaturisation in Pressure and Electroendosmotically Driven Liquid Chromatography: Some Theoretical Consideration.Chromatographia, 1987,24: 135–143.
Knox J H, Grant I H. Electrochromatography in Packed Tubes Using 1.5 to 50 μM Silica Gels and ODS Bonded Silica Gels.Chromatographia, 1991,32: 317–328.
Verbrugge M W, Hill R F. Ion and Solvent Transport in Ion-Exchange Membranes (1): A macrohomogeneous Mathematical Model.J Electroanal Soc, 1990,137: 886–893.
Verbrugge M W, Hill R F. Ion and Solvent Transport in Ion-Exchange Membranes (1): A Radiotracer Study of the Sulfuric-Acid, Nafion-117 System.J Electroanal Soc, 1990,137: 893–899.
Verbrugge M W. Methanol Diffusion in Perfluorinated Ion-Exchange Membranes.J Electroanal Soc, 1989,136: 417–423.
Jiang J H, Wu B L, Cha C S,et al. Application of SPE Composite Microlectrodes to the Study of the Spillover of Adsorbed Species on Electrode Surfaces.J Electroanal Chem, 1998,445: 13–16.
Jiang J H, Wu B L, Cha C S. SPE Composite Microdisk Electrodes Used in Organic Electrosynthesis.J Electroanal Chem, 1996,417: 89–93.
Tu W Y, Liu W J, Cha C S,et al. Study of the Powder/Membrane Interface by Using the Powder Microelectrode Technique I: the Pt-Black/Nafion Interfaces.Electrochimica Acta, 1998,43: 3731–3739.
Yik Y F, Li S F Y. Capillary Electrophoresis with Electrochemical Detection.Trends in Anal Chem, 1992,11: 325–332.
Kappes T, Hauser P C. Electrochemical Detector Methods in Capillary Electrophoresis and Applications to Inorganic Species.J Chromatography A 1999,834: 89–101.
Matysik F M. Improved End-Column Amperometric Detection for Capillary Electgrophoresis.J Chromatography A, 1996,742: 229–234
Ye J, Baldwin R P. Amperometric Detection in Capillary Electrophoresis with Normal Size Electrodes.Anal Chem, 1993,65: 3525.
Johnson D C, Lacourse W R. Liquid Chromatography with Pulsed Electrochemical Detection.Anal Chem, 1990,62: 589A-597A.
Ewing A G, Mesaros J M, Gavin P F. Electrochemical Detection in Microcolumn Separations.Anal Chem, 1994,66: 527A-536A.
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation item: Support by the Foundation of Electroanalytical Chemistry Open Lab (203231510), Changchum, China.
Biography: Fang Cheng (1971-), male, Lecture, Ph. D, research direction: Electroanalysis.
Rights and permissions
About this article
Cite this article
Cheng, F., Bing-Iiang, W., Wu-ming, Z. et al. Separation of dopamine and epinephrine by a novel electrophoresis technique with nafion membrane as separation column. Wuhan Univ. J. Nat. Sci. 9, 102–104 (2004). https://doi.org/10.1007/BF02912728
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02912728