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A rapid method for obtaining specific antibodies to mouse α1-fetoprotein

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Ricerca in clinica e in laboratorio

Summary

Rabbit antiserum to whole mouse amniotic fluid can be made α1-fetoprotein-specific byin vivo absorption into adult mice. The technique is easy to perform, with minimum training, and provides an average of 0.8–1.2 ml of specific antiserum in an absorption time of 5 min. The unfractionated absorbed antiserum, when precipitated with glutaraldehyde, can be used as an immunoabsorbent to purify α1-fetoprotein from whole amniotic fluid, yielding an average of 100 μg of α1-fetoprotein/ml of immunoabsorbent. The isolated protein appears antigenically pure when analyzed by SDS-PAGE.

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References

  1. Avrameas S., Ternynck T.: The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoabsorbents — Immunochemistry6, 53, 1969.

    Article  PubMed  CAS  Google Scholar 

  2. Celada F., Natali P. G., Radojkovic J.: Anin vitro immunoenzymatic assay of tumor antigens in the mouse with β-galactosidase — J. Immunol.117, 904, 1976.

    PubMed  CAS  Google Scholar 

  3. Laemmli U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 — Nature (Lond.)227, 680, 1970.

    Article  CAS  Google Scholar 

  4. McConahey P. J., Dixon F. J.: Method of trace iodination of proteins for immunologic studies — Int. Arch. Allergy29, 185, 1966.

    PubMed  CAS  Google Scholar 

  5. Mizejewski G. J., Simon R., Vonnegut M.: Purification of α1-fetoprotein from mouse amniotic fluid by gel-entrapped antibody filtration — J. immunol. Meth.31, 333, 1979.

    Article  CAS  Google Scholar 

  6. Natali P. G., Pellegrino M. A., Walker L., Ferrone S., Reisfeld R. A.: Antibody-coated protein-A bearingStaphylococcus aureus: a versatile and stable immune reagent — J. immunol. Meth.25, 255, 1979.

    Article  CAS  Google Scholar 

  7. Ruoslahti E., Seppälä M.: α1-fetoprotein in cancer and fetal development — Advanc. Cancer Res.29, 276, 1979.

    Google Scholar 

  8. Scheidegger J. J.: Une microméthode de l’immunoélectrophorèse — Int. Arch. Allergy7, 103, 1955.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratorio di Immunologia, Istituto Regina Elena per lo Studio e la Cura dei Tumori, Viale Regina Elena 291, 00161, Roma, Italia

    Pier Giorgio Natali & Maria Rita Nicotra

Authors
  1. Pier Giorgio Natali
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  2. Maria Rita Nicotra
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Additional information

This study was supported byConsiglio Nazionale delle Ricerche (CNR), Roma, Italy, Finalized CNR Project ‘Control of Tumour Growth’, grant no. 81.01326.96.

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Natali, P.G., Nicotra, M.R. A rapid method for obtaining specific antibodies to mouse α1-fetoprotein. La Ricerca Clin. Lab. 12, 479–484 (1982). https://doi.org/10.1007/BF02909399

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  • DOI: https://doi.org/10.1007/BF02909399

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