Summary
Vascular endothelial cell factor VIII/von Willebrand factor antigen (FVIII/vWF Ag) of normal and disordered gastric tissues was studied with staphyloceocus protein A-gold (PAG) labelling followed by photochemical silver reaction. FVIII/vWF Ag was localized clearly in the tissue fixed with various common fixatives and embedded in paraffin without enzyme treatment. The most satisfactory staining and the least nonspecific background were observed in the tissues fixed with Zamboni’s and Bouin’s solutions. The staining reaction could be enhanced, if the sections were preteateed with trypsin and subtilisin. Under the electron microscope, the gold particles were found over the Weibel-Palade bodies of vascular endothelial cells in the tissues fixed either in Zamboni’s solution or in Zamboni’s solution-osmium tetroxide, and embedded either with Lowicryl K4M or With Epon 812. It has been proved to be a better technique in investigation of FVIII/vWF Ag in vascular endothelial cells.
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References
Gitschier T, et al. Characterization of the human factor VIII gene. Nature 1984; 312:326–30.
Hamer RJ, et al. Factor VIII binds to von Willebrand factor via its M-80,000 light chain. Eur J Biochem 1987; 166:37–43
Wagner DD, et al. -Immunolocalization of von Willebrand protein in Weibel-Palade bodies of human endothelial cells J Cell Biol 1982; 95:355–60.
Kagawa H, Fujimoto S. Electron microscopic and immunocytochemical analysis of Weibel-Palade bodies in the human umbilical vein during pregnancy. Cell Tissue Res 1987:249:577–63.
Warhol MJ, Sweet JM. The ultrastructural localization of von Willebrand factor in endothelial cells. Am J Pathol 1984;117:310–5.
Roth J, et al. Enhancement of structural preservation and immunocytochemical staining in low temperature-embedded pancreatic tissue. J Histochem Cytochem 1981; 29:663–71.
Horisberger M, Rosset J. Colloidal gold, a useful marker for transmission and scanning electron microscopy. J Histochem Cytochem 1977;15:295–305.
Solt JW, Geuze HJ. A new method of preparing gold probes for muitiple-labell. ing cytochemistry. Eur J Cell 1985; 38:87.
Weibel ER, Palade GE. New cyloplasmic components in arterial endothelia. J Cell Biol 1964; 23:101–12.
Sengal A, Stoebner P. Golgi origin of tubular inclusions in endothelial cells. J Cell Biol 1970;44:223–8.
Curran RC, Gregory J. The unmasking of antigens in paraffin sections of tis sue by trypsin. Experimentia 1977; 33:1400–5.
Bloom AL, et al. Factor VIII on the vascuarl intima: possible importance in haemostasis and thrombosis. Nature (London) 1973; 241:217–23.
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Shi-liang, S., Yu-hui, X. & Zhong-bi, W. Immunohistochemical localization of vascular endothelial cell factor viii/von Willebrand factor antigen in human normal and disordered gastric tissues. Journal of Tongji Medical University 9, 193–198 (1989). https://doi.org/10.1007/BF02909082
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DOI: https://doi.org/10.1007/BF02909082