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The unmasking of antigens in paraffin sections of tissue by trypsin

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Summary

Paraffin sections of optimally fixed tissues often fail to react immunohistochemically. Short (15–30 min) exposure of the sections to 0.1% trypsin solution proves to be a very effective method of ‘unmasking’ the antigens.

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References

  1. R. C. Curran and E. L. Jones, Clin. exp. Immun.28, 103 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. S. Huang, H. Minassian and J. D. More, Lab. Invest.35, 383 (1976).

    CAS  PubMed  Google Scholar 

  3. A. R. Maunsbach, J. ultrastruct. Res.15, 242 (1966).

    Article  CAS  PubMed  Google Scholar 

  4. A. J. Garvin, S. S. Spicer and P. E. McKeever, J. exp. Med.139, 457 (1974).

    Article  Google Scholar 

  5. Dakopatts (Denmark) Code No. 10-090, 21-090 and Z113.+ DIFCO, 1∶250.

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Authors and Affiliations

  1. Department of Pathology, Medical School, B15 2TJ, Birmingham, England

    R. C. Curran & J. Gregory

Authors
  1. R. C. Curran
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  2. J. Gregory
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Curran, R.C., Gregory, J. The unmasking of antigens in paraffin sections of tissue by trypsin. Experientia 33, 1400–1401 (1977). https://doi.org/10.1007/BF01920206

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  • DOI: https://doi.org/10.1007/BF01920206

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