Skip to main content
SpringerLink
Log in

Immuno-electron localization of DNA in chondriolites ofSaccharomyces cerevisiae mitochondria

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Under electron microscope, the matrix of sectioned mitochondria exhibits ribosomes and an oval, electron-transparent zone which is devoid of ribosomes and is named chondriolite. Fine fibers or clumps of an electrondense material appeared in this zone after several fixation and contrasting steps and were identified with mitochondrial DNA by cytologists. To verify this assumption, we labeled DNA by a monoclonal antibody and a secondary antibody coupled to immunogold. The label was observed in the nucleus and in the chondriooite zone of sectioned mitochondria. Because the ultrastructure of chondriolites resembles that of nucleoids of prokaryotes, we suggest the term mitochondrial nucleoid for the zone of mitochondrial matrix devoid of ribosomes and containing DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Benoist P., Feau P., Pliss A., Voříšek J., Antonelli R., Raška I., Denis-Duphil M.: The yeast Ura2 protein that catalyses the first two steps of pyrimidines biosynthesis accumulates not in the nucleus but in the cytoplasm, as shown by immunocytochemistry and Ura2-green fluorescent protein mapping.Yeast 16, 1299–1312 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Borst P., Grivell L.A.: The mitochondrial genome of yeast.Cell 15, 705–723 (1978).

    Article  PubMed  CAS  Google Scholar 

  • Carlile M.J.: Prokaryotes and eukaryotes: strategies and successes.Trends Biochem. Sci. 7, 128–130 (1982a).

    Article  CAS  Google Scholar 

  • Carlile M.J.: Coding capacity of yeast mitochondrial DNA.Trends Biochem. Sci. 7, 281 (1982b).

    Article  Google Scholar 

  • Leger-Silvestre I., Noaillac-Depeyre C., Faubladier M., Gas N.: Structural and functional analysis of the nucleolus of the fission yeastSchizosaccharomyces pombe.Eur. J. Cell. Biol. 72, 13–23 (1997).

    PubMed  CAS  Google Scholar 

  • Matile Ph., Moor H., Robinow C.F.: Yeast cytology. The mitochondria, pp. 260–271 in A.H. Rose, J.S. Harrison (Eds.):The Yeasts, Vol. 1. Biology of Yeasts. Academic Press, London-New York 1969.

    Google Scholar 

  • Nagley P., Novitski C.E.: Coding capacity of yeast mitochondrial DNA.Trends Biochem. Sci. 7, 281 (1982).

    Article  Google Scholar 

  • Raška I., Dundr M., Koberna K., Melčák I., Risueno M.C., Torok I.: Does the synthesis of ribosomal DNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal.J. Struct. Biol. 114, 1–22 (1995).

    Article  PubMed  Google Scholar 

  • Rouillard J.M., Brendolise C., Lacroute F.: Rna14p, a component of the yeast nuclear cleavage/polyadenylation factor I, is also localized in mitochondria.Mol. Gen. Genet. 262, 1103–1112 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Stevens B.: Mitochondrial structure, pp. 471–504 in J.N. Strathern, E.W. Jones, J.R. Broach (Eds):The molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, New York 1981.

    Google Scholar 

  • Voříšek J., Pazlarová J., Hervé G.: Ultracytochemical localization of dihydroorotate dehydrogenase in mitochondria and vacuoles ofSaccharomyces cerevisae.Folia Microbiol. 38, 59–67 (1993).

    Article  Google Scholar 

  • Voříšek J., Gas N., Denis-Duphil M.: Cerium-based ultracytochemical localization of aspartate transcarbamylase activity in the cell membrane complex ofSaccharomyces cerevisiae.Micron 28, 221–230 (1997).

    Article  PubMed  Google Scholar 

  • Voříšek J., Noaillac-Depeyre J., Denis-Duphil M.: Life-cycle-dependent changes of aspartate carbamoyltransferase localization in membranes ofSaccharomyces cerevisiae—centrifugal elutriation and ultracytochemical study.Folia Microbiol. 44, 289–294 (1999).

    Article  Google Scholar 

  • Williamson D.H., Fennell D.H.: Visualization of yeast mitochondrial DNA with the fluorescent stain “DAPI”.Meth. Enzymol. 56, 728–733 (1979).

    Article  PubMed  CAS  Google Scholar 

  • Yotsuyanagi Y.: Études sur le chondriome de la levure. I. Variation de l'ultrastructure du chondriome au cours du cycle de la croissance aérobie.J. Ultrastruct. Res. 7, 121–140 (1962).

    Article  PubMed  CAS  Google Scholar 

  • Yotsuyanagi Y.: Fibrous component of yeast mitochondria.J. Ultrastruct. Res. 98, 254–266 (1988).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of Czech Republic, 142 20, Prague, Czechia

    J. Voříšek

  2. Faculty of Science, Charles University, 120 00, Prague, Czechia

    O. Toman

Authors
  1. J. Voříšek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. Toman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Voříšek.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Voříšek, J., Toman, O. Immuno-electron localization of DNA in chondriolites ofSaccharomyces cerevisiae mitochondria. Folia Microbiol 45, 239–242 (2000). https://doi.org/10.1007/BF02908951

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02908951

Keywords

Search

Navigation