Abstract
Chromosome III in a haploid Saccharomyces cerevisiae strain has been previously substituted for by its homeologue from S. carlsbergensis. With this chromosome substitution line the two homeologous chromosomes were shown to undergo crossing over only in a limited region, and to differ in nucleotide sequence at theHIS4 locus. In the present study the S. carlsbergensis chromosome III was compared to its S. cerevisiae homeologue at several additional loci.
Cloned DNA from the S. cerevisiae lociHML, HIS4, LEU2, MAT andSUP-RL1 was used as hybridization probes in the analysis of nucleotide sequence homology at these loci andHMR. Virtually no differences were detected atSUP-RL1 andHMR, located in the region where the two chromosomes recombine, whereas considerable differences were found in the non-recombining part. The data are consistent with the assumption thatHML, MAT andHMR of the S. carlsbergensis chromosome are organized as in S. cerevisiae Segments X and Z1, which are involved in mating type interconversion, were closely homologous to their S. cerevisiae counterparts, whereas Y α as well as sequences outside theHML andMAT cassettes were substantially different.
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Abbreviations
- bp:
-
base pair
- kb:
-
kilobase pair
References
Alwine, J. C., D. J. Kemp &G. R. Stark: Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethylpaper and hybridization with DNA probes. Proc. Nat. Acad. Sci. USA 74, 5350–5354 (1977)
Astell, C. R., L. Ahlstrom-Jonasson, M. Smith, K. Tatchell, K. A. Nasmyth &B. D. Hall: The sequence of the DNAs coding for the mating-type loci of Saccharomyces cerevisiae. Cell 27, 15–23 (1981)
Birnboim, H. C. &J. Doly: A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl. Acids Res. 7, 1513–1523, (1979)
Brandriss, M. C., J. W. Stewart, F. Sherman &D. Botstein: Substitution of serine caused by a recessive lethal suppressor in yeast. J. Mol. Biol. 102, 467–476 (1976)
Chinault, A. C. &J. Carbon: Overlap hybridization screening: Isolation and characterization of overlapping DNA fragments surrounding theleu2 gene on yeast chromosome III. Gene 5, 111–126 (1979)
Denhardt, D. T.: A membrane-filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23, 641–646 (1966)
Dobson, M. J., S. M. Kingsman &A. J. Kingsman: Sequence variation in theLEU2 region of theSaccharomyces cerevisiae genome. Gene 16, 133–139 (1981)
Dutcher, S. K.: Internuclear transfer of genetic information inkarl-1/KAR1 heterokaryons inSaccharomyces cerevisiae. Mol. Cell. Biol. 1, 245–253 (1981)
Guidelines for research involving recombinant DNA molecules. Federal Register U. S. 47 (77), 17180–17198 (1982)
Haber, J. E., D. W. Mascioli &D. T. Rogers: Illegal transposition of mating-type genes in yeast. Cell 20, 519–528 (1980)
Herskowitz, I. &Y. Oshima: Control of cell type inSaccharomyces cerevisiae: Matingtype and mating-type interconversion. In: The Molecular Biology of the Yeast Saccharomyces. J. N. Strathern, E. W. Jones and J. R. Broach, eds. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp 181–209 (1981)
Hicks, J., J. N. Strathern &A. J. S. Klar: Transposable mating type genes inSaccharomyces cerevisiae. Nature 282, 478–483 (1979)
Holmberg, S., M. C. Kielland-Brandt, T. Nilsson-Tillgren &J. G. L. Petersen: Molecular characterization of threehis4 deletion mutants in Saccharomyces cerevisiae. Carlsberg Res. Commun. 44, 283–288 (1979)
Holmberg, S., J. G. L. Petersen, T. Nilsson-Tillgren &M. C. Kielland-Brandt: Molecular characterization of a Saccharomyces plasmid containing theHIS4 gene. Carlsberg Res. Commun. 44, 269–282 (1979)
Jeffreys, A. J. &R. A. Flavell: A physical map of the DNA regions flanking the rabbit β-globin gene. Cell 12, 429–439 (1977)
Kielland-Brandt, M. C., T. Nilsson-Tillgren, S. Holmberg, J. G. L. Petersen &B. A. Svenningsen: Transformation of yeast without the use of foreign DNA. Carlsberg Res. Commun. 44, 77–87 (1979)
Klar, A. J. S., J. McIndoo, J. N. Strathern &J. B. Hicks: Evidence for a physical interaction between the transposed and the substituted sequences during mating type gene transposition in yeast. Cell 22, 291–298 (1980)
Klar, A. J. S., J. N. Strathern, J. R. Broach &J. B. Hicks: Regulation of transcription in expressed and unexpressed mating type cassettes of yeast. Nature 289, 239–244 (1981)
Klar, A. J. S., J. B. Hicks &J. N. Strathern: Directionality of yeast mating-type interconversion. Cell 28, 551–561 (1982)
Mortimer, R. K. &D. Schild: Genetic map ofSaccharomyces cerevisiae. Microbiol. Rev. 44, 519–571 (1980)
Nasmyth, K. A. &K. Tatchell: The structure of transposable yeast mating type loci. Cell 19, 753–764 (1980)
Nasmyth, K. A., K. Tatchell, B. D. Hall, C. Astell &M. Smith: Physical analysis of mating-type loci inSaccharomyces cerevisiae. Cold Spring Harbor Symp. Quant. Biol. 45, 961–981 (1981)
Nasmyth, K. A., K. Tatchell, B. D. Hall, C. Astell &M. Smith: A position effect in the control of transcription at yeast mating type loci. Nature 289, 244–250 (1981)
Nilsson-Tillgren, T., J. G. L. Petersen, S. Holmberg &M. C. Kielland-Brandt: Transfer of chromosome III duringkar mediated cytoduction in yeast. Carlsberg Res. Commun. 45, 113–117 (1980)
Nilsson-Tillgren, T., C. Gjermansen, M. C. Kielland-Brandt, J. G. L. Petersen &S. Holmberg: Genetic differences between Saccharomyces carlsbergensis and S. cerevisiae. Analysis of chromosome III by single chromosome transfer. Carlsberg Res. Commun. 46, 65–76 (1981)
Olson, M. V., G. S. Page, A. Sentenac, P. W. Piper, M. Worthington, R. B. Weiss &B. D. Hall: Only one of two closely related yeast suppressor tRNA genes contains an intervening sequence, Nature 291, 464–469 (1981)
Ratzkin, B. &J. Carbon: Functional expression of cloned yeast DNA inEcherichia coli. Proc. Nat. Acad. Sci. USA 74, 487–491 (1977)
Rigby, P. W. J., M. Dieckmann, C. Rhodes &P. Berg: Labeling deoxyribonucleic acid to high specific activityin vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113, 237–251 (1977)
Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)
Storms, R. K., J. B. McNeil, P. S. Khandekar, G. An, J. Parker &J. D. Friesen: Chimeric plasmids for cloning of deoxyribonucleic acid sequences inSaccharomyces cerevisiae. J. Bacteriol. 140, 73–82 (1979)
Strathern, J. N. &I. Herskowitz: Asymmetry and directionality in production of new cell types during clonal growth: the switching pattern of homothallic yeast. Cell 17, 371–381 (1979)
Strathern, J. N., C. S. Newlon, I. Herskowitz &J. B. Hicks: Isolation of a circular derivative of yeast chromosome III: Implications for the mechanism of mating type interconversion. Cell 18, 309–319 (1979)
Strathern, J. N., E. Spatola, C. McGill &J. B. Hicks: Structure and organization of transposable mating type cassettes inSaccharomyces yeasts Proc. Nat. Acad. Sci. USA 77, 2839–2843 (1980)
Sutcliffe, J. G.: pBR 322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucl. Acids Res. 5, 2721–2728 (1978)
Wahl, G. M., M. Stern &G. R. Stark: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc. Nat. Acad. Sci. USA 76, 3683–3687 (1979)
Yang, R. C.-A., J. Lis &R. Wu: Elution of DNA from agarose gels after electrophoresis. In: Methods in Enzymology, R. Wu ed. Academic Press, New York, 68, 176–182 (1979)
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Holmberg, S. Genetic differences between Saccharomyces carlsbergensis and S. cerevisiae II. Restriction endonuclease analysis of genes in chromosome III. Carlsberg Research Communicati 47, 233–244 (1982). https://doi.org/10.1007/BF02907875
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DOI: https://doi.org/10.1007/BF02907875