Abstract
Tobacco cv. Mamont was transformed by leaf disc method with A.tumefaciens C58C1 (pRiA4b) (pGA472). Transformed leaf segments were cultivated on medium with kanamycin as a selection agent, but without phytohormones. Transformed tissues grew as roots which later regenerated plants. Kanamycin in the medium ensured that only doubly transformed tissues, which contain in their genome both pRi T-DNA as well as pGA472 chimeric kanamycin resistance gene were recovered. Regenerated plants showed clear-cut morphological deviations, but only a slight increase of the auxin content. Kanamycin resistance in the progeny segregated in the Mendelian ratio 3: 1 or 2: 1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ackermann, C.: Pflanzen ausAgrobacterium rhizogenes-tumoren an Nicotiana tabacum. — Plant Sci. Lett.8: 23–30,1977.
Akiyoshi, D. E., Morris, R. O., Binz, R., Mischke, B. S., Kosuge, T., Garfinkel, D. J., Gordon, M. P., Nester, E. W.: Cytokinin-auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. — Proc. nat. Acad. Sci. USA80: 407–411, 1983.
An, G., Watson, B. P., Stachel, S., Gordon, M. P., Nester, E. W.: New cloning vehicles for transformation of higher plants. — EMBO J.4: 277–284, 1985.
Cardarelli, M., Spano, L., Mariotti, P., Mauro, M. L., VanSluiss, M. A., Costanttno, P.: The role of auxin in hairy root induction. — Mol. gen. Genet.208: 457–463, 1987.
Durand-Tardif, M., Broglie, R., Slightom, J., Tepfer, D.: Structure and expression of Ri T-DNA fromAgrobacterium rhizogenes in Nicotiana tabacum. — J. mol. Biol.186: 557–564, 1985.
Eder, J., Rovenská, J., Kutáček, M., Čermák, V.: HPLC analysis of indole compounds in Agrobacterium and transformed tobacco cells. — In:Kutáček, M.,Bandurski, R. S.,Krekule, J. (ed.): Physiology and Biochemistry of Auxins in Plants. (Proc. Int. Conf. Liblice.) Pp. 389–390. Academia, Praha + SPB Academic Publishing, The Hague1988.
Filetici, P., Spano, L., Costanttno, P.: Conserved region in the T-DNA of differentAgrobacterium rhizogenes root inducing plasmids. — Plant mol. Biol.9:19–26,1987.
Horsch, R., Fry, J., Hoffmann, N., Eichholz, D., Wallroth, M, Rogers, S., Fraley, R.: A simple and general method for transferring genes into plants. — Science227: 1229–1231, 1985.
Koplow, J., Byrne, M. C., Jen, G., Tempé, J., Chilton, M. D.: Physical map ofAgrobacterium rhizogenes strain 8196 virulence plasmid. — Plasmid11:17–27, 1984.
Maniatis, T., Frttsch, E. E., Sambrook, J.: Molècular Cloning: a Laboratory Manual. -Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1982.
Müller, A. J., Mendel, R. R., Schiemann, J., Simoens, G., Iipnzé, D.: High meiotic stability of foreign gene introduced into tobacco by Agrobacterium-mediated transformation. — Mol. gen. Genet.207:171–175,1987.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. —Plant Physiol.15: 473–497, 1962.
Petit, A., David, G., Dahl, G., Ellis, J. G., Guyon, P., Casse-Delbart, F., Tempe, J.: Further extension of the opine concept: plasmids inAgrobacterium rhizogenes cooperate for opine degradation. — Mol. gen. Genet.190: 204–214, 1983,
Shahin, E. A., Sukhapinda, K., Simpson, R.B., Spivey, R.: Transformation of cultivated tomato by a binary vector inAgrobacterium rhizogenes: transgenic plants with normal phenotypes harbor binary vector T-DNA, but no Ri plasmid T-DNA. — Theor. appl. Genet.72: 770–777, 1986.
Simpson, J., Timko, M. P., Cashmoore, A. R., Schell, J., Van Montagu,M., Herrera-Estrella, L.: Light inducible and tissue specific expression of a chimsric gene under control of 5′-flanking sequence of a pea chlorophyll a/b binding protein. — EMBO J.4: 2723–2729, 1985.
Slightom, J. L., Durand-Tardif, M., Jouanin, L.,Tepfer, D.: Nucleotide sequence analysis of Tl-DNA ofAgrobacterium rhizogenes agropine type plasmid. — J. biol. Chem.261: 108–121, 1986.
Taylor, B., Powell, A.: Isolation of plant DNA. — BRL Focus3:1–5, 1983.
White, F. F., Taylor, B. H., Huffman, G. A., Gordon, M. P., Nester, E. W.: Molecular and genetic analysis of the transferred DNA region of the root-inducing plasmid of Agrobacteriumrhizogenes. — J. Bacteriol.164: 33–44, 1985.
Wullems, G. J., Molendijk, L., Ooms, G., Schilperoort, R. A.: Retention of tumor markers in F1 progeny plants in invitro induced octopine and nopaline tumor tiśsues. — Cell24: 719–727, 1981.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
OndŘrej, M., Eder, J., Hrouda, M. et al. Free auxin level and inheritance of introduced markers in tobacco transformed by binary vector eased on A4 Ri plasmid. Biol Plant 31, 286–291 (1989). https://doi.org/10.1007/BF02907290
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02907290