Abstract
Among theeceriferum locicer-c,-q and-u control three different steps in the pathway leading to β-diketones, hydroxy-β-diketones and esterified alkan-2-ols. Chemical analyses of the wax on spikes of wild type and representative single and apparent multiple mutants demonstrated that such mutants do not affect the composition of the other wax lipids and confirmed the earlier deduced sites of action ofcer-c,-q and-u. Each pair of the three markerscer-c 3,-q35 and-u 58 was found by test cross analysis to be closer together than 0.025 map units. This deduction was derived from the failure of the markers to recombine in the 26,933 gametes tested. The obtained results are expected 95% of the time if the actual distance between any pair is approximately 0.0012 map units. The linear sequence of the three markers could not be determined. The combined results lead to the suggestion that thecer-cqu region is more likely to represent a multifunctional gene than a gene cluster.
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Abbreviations
- DEPH:
-
di-2-ethylhexylphthalate
- GLC:
-
gas liquid chromatography
- TLC:
-
thin layer chromatography
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von Wettstein-Knowles, P., Søgaard, B. Thecer-cqu region in barley: Gene cluster or multifunctional gene. Carlsberg Res. Commun. 45, 125–141 (1980). https://doi.org/10.1007/BF02906514
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DOI: https://doi.org/10.1007/BF02906514